| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,322,183 | G→A | 69.6% | L297L (CTG→TTG) | sbp ← | sulfate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,322,183 | 0 | G | A | 69.6% | 96.7 / 50.7 | 105 | L297L (CTG→TTG) | sbp | sulfate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (24/8); new base A (49/24); total (73/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAATGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGTTTT > minE/2322122‑2322245 | aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:247521/69‑1 (MQ=255)aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2516847/69‑1 (MQ=255)aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:3041245/69‑1 (MQ=255)aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:3017099/69‑1 (MQ=255)aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2711791/69‑1 (MQ=255)aaaTGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGa < 1:2816767/69‑1 (MQ=255) gcgcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCg < 1:1480694/68‑1 (MQ=255) gcgcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCg < 1:1895884/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1682939/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:859473/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:3057187/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2890327/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2836061/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2621403/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2011171/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2603125/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2080950/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:2118741/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:225763/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1075234/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:702572/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:6434/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1233818/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:3396806/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1238086/68‑1 (MQ=255) gcTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:522456/68‑1 (MQ=255) cTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:390935/67‑1 (MQ=255) tCGTCCAGCCGCCGAACTCTTCATCAATCGTGAATAACTTCCATTTTGGAAACGCATTTTCGTa < 1:1360283/64‑1 (MQ=255) gTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1948383/62‑1 (MQ=255) gccgAACTCTTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1653276/54‑1 (MQ=255) gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACttt > 1:3167351/1‑55 (MQ=255) gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:3258926/1‑69 (MQ=255) gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:2144853/1‑69 (MQ=255) gAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGc > 1:2286166/1‑69 (MQ=255) ctctTCATCAATGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTa < 1:1871632/48‑1 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:504266/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:338918/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:574825/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:337227/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:3288167/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:725616/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:850133/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:2450040/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:1917545/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:198193/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:15297/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:2045432/1‑69 (MQ=255) ctTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:212994/1‑69 (MQ=255) catcatcaATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgc > 1:1196247/3‑69 (MQ=255) ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAg > 1:1636093/1‑62 (MQ=255) ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgcg > 1:2856026/1‑69 (MQ=255) ttCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTcgcg > 1:1110561/1‑69 (MQ=255) tGGTGACTAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:6493/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTc > 1:2912966/1‑44 (MQ=38) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTAcc > 1:2315518/1‑50 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTc > 1:1240655/1‑52 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1806611/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:39395/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1818960/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1835777/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:3185564/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:3265779/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:441630/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:284567/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2841621/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:384198/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2706807/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2632079/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2588860/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1953841/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2565440/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2538715/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2482081/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:1000259/1‑69 (MQ=255) tGGTGAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACGgt > 1:2379513/1‑69 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTcg > 1:2326908/1‑55 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1009678/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:412118/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2226852/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1041631/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:447888/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:469930/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:480168/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1075314/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1143162/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1249120/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1410032/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1511712/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1565396/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1573805/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1856245/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:1877970/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2765866/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2571446/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2536144/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2188080/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2446387/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2341870/1‑63 (MQ=255) gAATAACTTCAATTTTGGAAACGCATTTTCGTACTTTTTCGCTACCTCAGCGTCGCGCGGACg > 1:2299495/1‑63 (MQ=255) aCTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGtttt > 1:1576974/1‑69 (MQ=255) | AAATGCTCTTTTTGCGCTTTCGTCCAGCCGCCGAACTCTTCATCAATGGTGAATAACTTCAGCTTTGGAAACGCATTTTCGTACTTTTTCGCCACCTCAGCGTCGCGCGGACGGTAGTAGTTTT > minE/2322122‑2322245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |