Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,323,249 | G→A | 57.4% | intergenic (‑178/+142) | sbp ← / ← pfkA | sulfate transporter subunit/6‑phosphofructokinase I |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,323,249 | 0 | G | A | 57.4% | ‑1.4 / 44.8 | 61 | intergenic (‑178/+142) | sbp/pfkA | sulfate transporter subunit/6‑phosphofructokinase I |
Reads supporting (aligned to +/- strand): ref base G (6/20); new base A (14/21); total (20/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
TAAAAAGGCGGAAGAAATCCGCCTCATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCA > minE/2323183‑2323304 | tAAAAAGGCGGAAGAAATCCGCCTCATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGcgcg < 1:2426987/69‑1 (MQ=255) tAAAAAGGCGGAAGAAATCCGCCTCATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGcgcg < 1:3104344/69‑1 (MQ=255) tAAAAAGGCGGAAGAAATCCGCCTCATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGcgcg < 1:2991581/69‑1 (MQ=255) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAg < 1:1169212/69‑1 (MQ=255) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAg < 1:459250/69‑1 (MQ=255) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:183798/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:2121511/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1910081/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:2187319/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1001586/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:2516951/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:653476/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:2794479/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:286925/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:512868/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:3116008/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:3184931/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:417455/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:374011/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:37678/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:397391/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1273320/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1073919/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1408589/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCACAAAc < 1:1540068/48‑1 (MQ=17) cATATTGCTGACAAAGTGCGCTTTGTACATGCCGGATGCGGCACAAAc < 1:1554476/48‑1 (MQ=14) tGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTg > 1:1064894/1‑68 (MQ=255) gACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTTCaaa < 1:2620483/68‑1 (MQ=255) gACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCaaa < 1:1385809/68‑1 (MQ=255) gACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCaaa < 1:2125670/68‑1 (MQ=255) gACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCaaa < 1:1649136/68‑1 (MQ=255) aaaGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:180414/1‑68 (MQ=255) aaaGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:2871926/1‑68 (MQ=255) aaaGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1386749/1‑68 (MQ=255) aaaGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:958873/1‑68 (MQ=255) ctGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc < 1:2728806/67‑1 (MQ=255) ctGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc < 1:3306489/67‑1 (MQ=255) ctGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc < 1:698479/67‑1 (MQ=255) ctGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc < 1:971674/67‑1 (MQ=255) ctGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc < 1:1590798/67‑1 (MQ=255) tCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAg < 1:1236845/67‑1 (MQ=255) tCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAg < 1:1251682/67‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:3271499/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:1406755/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:3097940/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:893766/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:2266794/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:1587931/69‑1 (MQ=255) tGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa < 1:211262/69‑1 (MQ=255) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAAtt > 1:912549/1‑50 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:2480956/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:731359/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:995193/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:335623/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:3272161/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:2974263/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:2711163/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:2173340/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1595980/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1448103/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1247195/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1173726/1‑51 (MQ=14) tGCCGGATGCGGCACAAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTc > 1:1087660/1‑51 (MQ=14) ccGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCa > 1:1011510/1‑67 (MQ=255) | TAAAAAGGCGGAAGAAATCCGCCTCATATTGCTGACAAAGTGCGCTTTGTCCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGCAGAATTCA > minE/2323183‑2323304 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |