| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,325,765 | A→T | 27.1% | I107I (ATT→ATA) | cpxP ← | periplasmic protein combats stress |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,325,765 | 0 | A | T | 27.1% | 113.4 / 46.2 | 81 | I107I (ATT→ATA) | cpxP | periplasmic protein combats stress |
| Reads supporting (aligned to +/- strand): ref base A (30/29); new base T (9/13); total (39/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
GCTGCTCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGT > minE/2325701‑2325830 | gctgctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAAttt > 1:749216/1‑69 (MQ=255)gctgctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAAttt > 1:2220879/1‑69 (MQ=255)gctgctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAAttt > 1:2448932/1‑69 (MQ=255)gctgctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAAttt > 1:2927368/1‑69 (MQ=255)gctgctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAAttt > 1:30385/1‑69 (MQ=255) gctCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCt < 1:2616825/69‑1 (MQ=255) ccGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:1305876/68‑1 (MQ=255) ccGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3117549/68‑1 (MQ=255) ccGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:2779153/68‑1 (MQ=255) ccGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:2128146/68‑1 (MQ=255) tAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCAt < 1:1258858/69‑1 (MQ=255) aGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCAttttt < 1:764056/69‑1 (MQ=255) aGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCAttttt < 1:2314530/69‑1 (MQ=255) aGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCAttttt < 1:529809/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:1271196/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:419700/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:2385007/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:2467334/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:2070632/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:2543732/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:696255/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:1875422/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:738978/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:1739750/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:3046847/69‑1 (MQ=255) cGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCt < 1:1755378/52‑1 (MQ=255) gATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:654742/68‑1 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCt > 1:1150073/1‑69 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCt > 1:314911/1‑69 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCt > 1:570781/1‑69 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCt > 1:1998395/1‑69 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCt > 1:756101/1‑69 (MQ=255) aCATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATGTTTTCTGCCt > 1:1305999/1‑69 (MQ=255) cATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCtg > 1:2420977/1‑69 (MQ=255) cATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCtg > 1:315410/1‑69 (MQ=255) cATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCtg > 1:2110077/1‑69 (MQ=255) cATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCtg > 1:426294/1‑69 (MQ=255) cATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCtg > 1:1471540/1‑69 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3262454/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3257885/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:317610/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:862626/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3301342/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:566771/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3366266/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:2202244/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:2969596/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:1332993/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:3379487/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:1794863/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:266016/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:710509/1‑46 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:2085484/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:243543/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa < 1:692564/46‑1 (MQ=255) ttGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCa > 1:424385/1‑46 (MQ=255) ttGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTgcgcg > 1:3000473/1‑68 (MQ=255) ttGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTgcgcg > 1:821697/1‑68 (MQ=255) ttGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTgcgcg > 1:1244271/1‑68 (MQ=255) ttGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTgcgcg > 1:2456748/1‑68 (MQ=255) ttGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTgcgcg > 1:2369391/1‑68 (MQ=255) aCTTTTGCCATCTCAACCTGACGAGCTATTTGCTCATTCGCCATTTTTTCt < 1:1290450/51‑1 (MQ=255) tcAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCaaaa > 1:3341586/1‑69 (MQ=255) tcAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCaaa > 1:2719429/1‑68 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:2706590/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:1176144/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:3092081/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:1745466/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:185703/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:1089114/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:1914511/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATTTTTTC‑‑CCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt > 1:2186680/1‑69 (MQ=255) cGAGCTATTTGCTCATTCGCCATATTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCg > 1:3300445/1‑69 (MQ=255) aGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:1485589/69‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:683431/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:603343/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:1838565/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:1207072/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:3380205/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:993829/67‑1 (MQ=255) cAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTAATCAAAATTTTCTGCGGt < 1:1320926/67‑1 (MQ=255) aaTTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGt < 1:2951563/66‑1 (MQ=255) | GCTGCTCCGGCGTTAACAGGCGATACATTTGGTTGCGGACTTTTGCCATCTCAACCTGACGAGCAATTTGCTCATTCGCCATTTTTTCTGCCTGTGCGCGCACAGCGTTTTCATCAAAATTTTCTGCGGT > minE/2325701‑2325830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |