Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,330,847 G→A 100% S83S (AGC→AGT)  sodA ← superoxide dismutase, Mn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,330,8470GA85.2% 99.8 / 7.5 60S83S (AGC→AGT) sodAsuperoxide dismutase, Mn
Reads supporting (aligned to +/- strand):  ref base G (9/0);  new base A (23/28);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 2.36e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTT  >  minE/2330818‑2330894
                             |                                               
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:591624/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:589665/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:303495/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:354920/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:3300789/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:3300346/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:1943626/1‑69 (MQ=255)
gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg          >  1:3198300/1‑69 (MQ=255)
  gCCTTTATTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGt        >  1:6716/1‑69 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:2723433/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1076948/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:284271/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:293247/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:3053042/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:312818/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:3140018/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:352092/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:437112/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:496360/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:664314/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:832193/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:913014/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:2069617/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1444120/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1533945/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:2551696/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:160567/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1795426/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1172014/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:112748/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:2172874/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1117113/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1116504/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:2212838/68‑1 (MQ=255)
         ttCAGACCTTTCCAGAACAGACTGAGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1987705/68‑1 (MQ=255)
          tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:1870561/67‑1 (MQ=255)
          tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt  <  1:833593/67‑1 (MQ=255)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:788459/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:1238432/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:354271/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:571565/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:541782/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:1013281/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:531570/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:1272506/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:962073/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:467865/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:386199/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:384744/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:344744/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:3241988/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:3139275/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2070264/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2079209/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:3032297/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:3031064/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2945954/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2919504/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2315382/1‑46 (MQ=38)
                cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                 >  1:2747253/1‑46 (MQ=38)
                             |                                               
GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTT  >  minE/2330818‑2330894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑