| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,330,865 | G→A | 100% | G77G (GGC→GGT) | sodA ← | superoxide dismutase, Mn |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,330,865 | 0 | G | A | 81.1% | 92.5 / 12.4 | 63 | G77G (GGC→GGT) | sodA | superoxide dismutase, Mn |
| Reads supporting (aligned to +/- strand): ref base G (11/1); new base A (23/28); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGC > minE/2330818‑2330928 | gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:591624/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:589665/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:303495/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:354920/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:3300789/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:3300346/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:1943626/1‑69 (MQ=255)gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg > 1:3198300/1‑69 (MQ=255) gCCTTTATTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGt > 1:6716/1‑69 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:2723433/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1076948/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:284271/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:293247/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:3053042/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:312818/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:3140018/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:352092/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:437112/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:496360/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:664314/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:832193/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:913014/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:2551696/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1444120/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1533945/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:160567/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1795426/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:2069617/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1172014/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:2172874/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:112748/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1117113/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:2212838/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1116504/68‑1 (MQ=255) ttCAGACCTTTCCAGAACAGACTGAGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1987705/68‑1 (MQ=255) tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:1870561/67‑1 (MQ=255) tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt < 1:833593/67‑1 (MQ=255) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:788459/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:1238432/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:571565/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:541782/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:531570/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:1272506/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:467865/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:1013281/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:962073/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2079209/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2747253/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2315382/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2919504/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2945954/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:3031064/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:3032297/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:386199/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:2070264/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:3139275/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:3241988/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:344744/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:354271/1‑46 (MQ=38) cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg > 1:384744/1‑46 (MQ=38) tgGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTg > 1:2878990/1‑69 (MQ=255) tAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTg < 1:828902/68‑1 (MQ=255) tGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGc > 1:3065491/1‑69 (MQ=255) | GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGC > minE/2330818‑2330928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |