Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,330,874	G→A	71.9%	N74N (AAC→AAT)	sodA ←	superoxide dismutase, Mn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,330,874	0	G	A	71.9%	70.9 / 28.9	71	N74N (AAC→AAT)	sodA	superoxide dismutase, Mn
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base A (23/28);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAACCGGCAG  >  minE/2330818‑2330942                                                         |                                                                     gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:3300346/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:589665/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:591624/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:1943626/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:354920/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:303495/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:3300789/1‑69 (MQ=255) gtgCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACg                                                          >  1:3198300/1‑69 (MQ=255)   gCCTTTATTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGt                                                        >  1:6716/1‑69 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:3053042/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:312818/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:352092/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:293247/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:437112/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:284271/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:2723433/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:2551696/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:496360/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:3140018/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:2212838/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:2172874/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1076948/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:913014/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1116504/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:664314/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1117113/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1795426/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:160567/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1533945/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1444120/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:112748/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:2069617/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1172014/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:832193/68‑1 (MQ=255)          ttCAGACCTTTCCAGAACAGACTGAGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1987705/68‑1 (MQ=255)           tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:1870561/67‑1 (MQ=255)           tCAGACCTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTGCGCAGTACGGTTTTCtt                                                  <  1:833593/67‑1 (MQ=255)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:1013281/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:344744/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:788459/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:962073/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:354271/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:384744/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:386199/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:467865/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:531570/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:541782/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:571565/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:3241988/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:1272506/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2070264/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2079209/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:1238432/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2315382/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2747253/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2919504/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:2945954/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:3031064/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:3032297/1‑46 (MQ=38)                 cTTTCCAGAACAGACTGTGGTTAGCGTGACCACCAGCGTTATTgcg                                                                 >  1:3139275/1‑46 (MQ=38)                                  tgGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTg                         >  1:2878990/1‑69 (MQ=255)                                      tAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTg                      <  1:828902/68‑1 (MQ=255)                                           tGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGc                >  1:3065491/1‑69 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:3269228/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:9203/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:1246140/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:1120223/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:1407109/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:1448748/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:2005109/69‑1 (MQ=255)                                                    gCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:2940356/69‑1 (MQ=255)                                                      gttgttGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAAcc       <  1:2003882/67‑1 (MQ=255)                                                         gttgCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAACCGGCAg  >  1:574637/1‑69 (MQ=255)                                                         gttgCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAACCGGCAg  >  1:2086326/1‑69 (MQ=255)                                                         gttgCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAACCGGCAg  >  1:2459539/1‑69 (MQ=255)                                                         |                                                                     GTGCCTTTTTTCAGACCTTTCCAGAACAGGCTGTGGTTAGCGTGACCGCCAGCGTTGTTGCGCAGTACGGTTTTCTTGTCTGCTGGCAGCTGGTCCAGTTTGGTGATCAGCTCTTCAACCGGCAG  >  minE/2330818‑2330942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑