| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,332,248 | G→C | 32.2% | V37V (GTC→GTG) | fdhD ← | formate dehydrogenase formation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,332,248 | 0 | G | C | 32.2% | 122.6 / 82.8 | 112 | V37V (GTC→GTG) | fdhD | formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base G (39/36); new base C (15/21); total (54/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GGCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACG > minE/2332183‑2332304 | ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1055802/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3203175/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3175849/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:3104880/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1327199/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:2881504/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:17821/66‑1 (MQ=255)ggCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCcgcg < 1:1725919/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:973999/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:949410/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:2615626/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:216591/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:1847524/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCa < 1:1734413/66‑1 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:977555/1‑69 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:777597/1‑69 (MQ=255) gCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCt > 1:1078747/1‑69 (MQ=255) ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:3035243/69‑1 (MQ=255) ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:391185/69‑1 (MQ=255) ccATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTa < 1:478871/69‑1 (MQ=255) catcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCg > 1:710252/1‑69 (MQ=255) atcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGt > 1:3022054/1‑69 (MQ=255) atcaCCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGt > 1:610417/1‑69 (MQ=255) aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:974873/69‑1 (MQ=255) aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2850164/69‑1 (MQ=255) aaTGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:385291/69‑1 (MQ=255) tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:3287809/69‑1 (MQ=255) tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:808653/69‑1 (MQ=255) tGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:680257/69‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCGGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:539990/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3134569/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3191305/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2268757/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1006601/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:3098506/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2967941/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2658574/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:2632941/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:225588/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:705871/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:771072/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1983173/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:811280/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1888332/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1884677/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1870171/59‑1 (MQ=255) ttGTAGACCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTaa < 1:1560161/59‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1316242/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1696003/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:136695/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1387385/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2559635/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2255143/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:1985545/60‑1 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:2517550/1‑68 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:3017409/1‑68 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCAcgcc > 1:812522/1‑68 (MQ=255) gTAGACCAGCGCAACGGGAACTTCTTCCGCAACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2008070/60‑1 (MQ=255) tAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatc < 1:2613881/59‑1 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCa > 1:1949827/1‑68 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:464693/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:512428/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:3428126/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2265392/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:721774/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:3169403/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2372876/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:922837/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:1077253/1‑69 (MQ=255) aCCAGCGCAACGGGAACTTCTTCCGCCACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCAt > 1:2664817/1‑69 (MQ=255) ccAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat < 1:2429751/57‑1 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTg > 1:2114564/1‑41 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcatc > 1:2508973/1‑57 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1099102/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1531231/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:689395/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:155075/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2058070/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:1655956/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2561000/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2430316/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:362271/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2334305/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:3286630/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:3180180/1‑69 (MQ=255) cAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATaa > 1:2932071/1‑69 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2478045/1‑68 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:3025025/1‑68 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2360431/1‑68 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:3420725/1‑68 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2865729/1‑68 (MQ=255) aCGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAAt > 1:2117808/1‑68 (MQ=255) aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1170112/69‑1 (MQ=255) aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1311749/69‑1 (MQ=255) aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:1976033/69‑1 (MQ=255) aaCTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACg < 1:2098863/69‑1 (MQ=255) cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:279852/1‑42 (MQ=255) cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:2004453/1‑42 (MQ=255) cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:1413409/1‑42 (MQ=255) cttcttCCGCGACCTCATCTAACCGTGGGTGTTGTAAatcat > 1:2114733/1‑42 (MQ=255) | GGCGACGCCATCATCACCACATGCGAAATGCCGTTGTAGACCAGCGCAACGGGAACTTCTTCCGCGACCTCATCTAACCGTGGGTGTTGTAAATCATCACGCCGCCATAACTCAATTTGACG > minE/2332183‑2332304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |