| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,334,291 | C→G | 30.6% | V580V (GTC→GTG) | fdoG → | formate dehydrogenase‑O, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,334,291 | 0 | C | G | 30.6% | 165.0 / 84.3 | 121 | V580V (GTC→GTG) | fdoG | formate dehydrogenase‑O, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/50); new base G (26/11); total (60/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.09e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGA > minE/2334229‑2334353 | gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:938734/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:214170/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2203081/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:1923357/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:2788809/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:1314239/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:3102112/1‑69 (MQ=255)gTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATc > 1:447790/1‑69 (MQ=255) gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:2572183/1‑68 (MQ=255) gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1673502/1‑68 (MQ=255) gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1123520/1‑68 (MQ=255) gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:1553607/1‑68 (MQ=255) gCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGa > 1:135632/1‑68 (MQ=255) tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:1225997/1‑69 (MQ=255) tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2779816/1‑69 (MQ=255) tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2501252/1‑69 (MQ=255) tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:2791965/1‑69 (MQ=255) tCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCca > 1:138028/1‑69 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2421091/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:94509/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2411085/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:239232/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2181087/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3274371/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2599246/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:43196/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2867517/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3370006/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2887270/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:2970074/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3107038/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3152646/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3233747/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:14130/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa > 1:2041725/1‑69 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1282944/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1105178/69‑1 (MQ=255) cccGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:1227893/69‑1 (MQ=255) cGAACAAAAACAAAGTGATCGGTTGTCTGGCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3069008/67‑1 (MQ=255) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTAt > 1:2320308/1‑52 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1135172/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2736589/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2781325/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1343472/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2958475/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:3342141/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:3394846/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2104780/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:456776/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:465582/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:477556/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:549253/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:622798/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:775449/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1063014/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2689155/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2691943/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2124770/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:2276925/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1680172/1‑53 (MQ=25) acaaaaacaaaGTGATCGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1727682/1‑53 (MQ=25) acaaaaacaaaGTGATAGGCTGTCTGTCGAAGCTGAAATTCCTCGTGACTATc > 1:1022315/1‑53 (MQ=25) caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:700780/69‑1 (MQ=255) caaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactga < 1:2116289/69‑1 (MQ=255) aaaaacaaaGTGTTCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2449073/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3272354/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1471083/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2061423/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2677807/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1353794/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:908086/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:91015/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1766353/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2071884/69‑1 (MQ=255) aaaaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:2922932/69‑1 (MQ=255) aaaaacaaaGTGATCGGATGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:3217355/69‑1 (MQ=255) aaaaacaaaGCGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaacactgaa < 1:1284047/69‑1 (MQ=255) aaacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1235507/69‑1 (MQ=255) aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCa > 1:1191924/1‑46 (MQ=255) aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAcc > 1:1230281/1‑69 (MQ=255) aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc < 1:1810709/68‑1 (MQ=255) aacaaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAAc > 1:921091/1‑68 (MQ=255) aaaGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCcactgaa < 1:3412973/56‑1 (MQ=255) tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:843076/1‑69 (MQ=255) tGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACt > 1:1731415/1‑69 (MQ=255) tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:2355332/69‑1 (MQ=255) tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:900187/69‑1 (MQ=255) tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:213446/69‑1 (MQ=255) tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:1800910/69‑1 (MQ=255) tgtctgtcGAAACTGAAGTTCCTCGTCACCATCGAACCACTGAACACTGAAACCTCTAACTTCTGGCAg < 1:828525/69‑1 (MQ=255) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTTGCAGAACCACGGt < 1:2003894/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2217535/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2373874/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:847326/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:1258429/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:1272905/66‑1 (MQ=18) agcTGAAATTCCTCGTGACTATCGACCCGCTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGt < 1:2751002/66‑1 (MQ=18) gTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTg < 1:330967/67‑1 (MQ=255) ccTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGa > 1:681722/1‑69 (MQ=255) ccTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGa > 1:3042427/1‑69 (MQ=255) | GTTGCCTCATTCCCGAACAAAAACAAAGTGATCGGTTGTCTGTCGAAACTGAAGTTCCTCGTCACCATCGACCCACTGAACACTGAAACCTCTAACTTCTGGCAGAACCACGGTGAGCTGAACGA > minE/2334229‑2334353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |