| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,336,886 | C→T | 43.0% | L125L (CTG→TTG) | fdoI → | formate dehydrogenase‑O, cytochrome b556 subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,336,886 | 0 | C | T | 43.0% | 18.9 / 47.3 | 65 | L125L (CTG→TTG) | fdoI | formate dehydrogenase‑O, cytochrome b556 subunit |
| Reads supporting (aligned to +/- strand): ref base C (12/25); new base T (13/15); total (25/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
GACACCGGGCGTTATAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTTCTCAATCCCGGTGA > minE/2336820‑2336949 | gACACCGGGCGTTATAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTgtgctg > 1:1932546/1‑69 (MQ=255)gACACCGGGCGTTATAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgctg > 1:1293303/1‑69 (MQ=255)gACACCGGGCGTTATAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgctg > 1:1907169/1‑69 (MQ=255) ataACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAgcggc > 1:804889/1‑68 (MQ=255) ataACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAgcggc > 1:1910252/1‑68 (MQ=255) ataACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAgcggc > 1:1676408/1‑68 (MQ=255) ataACTTCGGTCAGAAATGAGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAgagga > 1:1933644/1‑67 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:1727216/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:2151632/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:282419/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:2211449/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:3164352/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:3276896/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:3297644/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:2772792/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:512159/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:653483/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:701942/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:117559/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTCCTGTTGCTGGTGAGCGGCGTg > 1:2646071/1‑68 (MQ=255) aCTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCAGGTCctgttgctg > 1:1685492/1‑56 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:2360844/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:2688084/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:2790516/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:2827806/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:3321765/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:538394/69‑1 (MQ=255) ttCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGAtt < 1:981962/69‑1 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTg > 1:1157191/1‑63 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:2237141/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:2977858/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:796991/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:2218338/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:3153827/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:3180581/1‑68 (MQ=255) aTGCGTTTTCTGGGCGGCGATTATTTTACTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTc > 1:1346296/1‑68 (MQ=255) tGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:225354/69‑1 (MQ=255) tGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:392234/69‑1 (MQ=255) tGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:1564487/69‑1 (MQ=255) tGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:3080611/69‑1 (MQ=255) tGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:1555000/69‑1 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:3161674/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:3431555/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:1766628/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:173776/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:1728667/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:2530390/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:2350957/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTctgct > 1:2264573/1‑37 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCt < 1:2608864/68‑1 (MQ=255) gCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGGGAGCGGCGTGATTATCTGGCGTCCt < 1:2006062/68‑1 (MQ=255) cGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTa < 1:1893319/69‑1 (MQ=255) cGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTa < 1:1619979/69‑1 (MQ=255) cGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTa < 1:2262967/69‑1 (MQ=255) gggCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTgcg < 1:50634/68‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:80329/69‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:811948/69‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:95222/69‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:229472/69‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:1894476/69‑1 (MQ=255) tattTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:1961974/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:388940/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:3174355/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:3173907/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:3116440/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:615183/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:657487/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:710984/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:2738229/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:2724387/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:264532/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:851902/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:904348/69‑1 (MQ=255) tattTTCCTGGTCCTGTTGCTGGTGAGAGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:1491424/69‑1 (MQ=37) tattTTCCTGCTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:3192857/69‑1 (MQ=25) attTTCCTGGTCCTGTTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTtctc < 1:2715418/68‑1 (MQ=255) tCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTTCTCAATCCCGGTGa < 1:1125768/68‑1 (MQ=255) | GACACCGGGCGTTATAACTTCGGTCAGAAATGCGTTTTCTGGGCGGCGATTATTTTCCTGGTTCTGCTGCTGGTGAGCGGCGTGATTATCTGGCGTCCTTATTTTGCGCCTGCTTTCTCAATCCCGGTGA > minE/2336820‑2336949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |