| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,337,184 | A→G | 39.6% | S13S (TCA→TCG) | fdhE → | formate dehydrogenase formation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,337,184 | 0 | A | G | 39.6% | 25.6 / 41.9 | 63 | S13S (TCA→TCG) | fdhE | formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base A (3/35); new base G (15/10); total (18/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAATTTATACA > minE/2337119‑2337248 | ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:2253635/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:950606/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:250543/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:2509444/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:2659579/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:1523696/1‑67 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGATCa > 1:1399603/1‑66 (MQ=255)ccGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAAGCCCGCAAGATGAGCTGGGTTCaa > 1:611044/1‑67 (MQ=255) cGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:2807687/1‑66 (MQ=255) cGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:895739/1‑66 (MQ=255) cGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCaa > 1:122461/1‑66 (MQ=255) aaGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCgaga < 1:982069/69‑1 (MQ=255) cAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACg < 1:1621057/69‑1 (MQ=255) cAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACg < 1:2312962/69‑1 (MQ=255) cAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACg < 1:231150/69‑1 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACg > 1:2258824/1‑61 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGt > 1:2483281/1‑62 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:291354/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:2872163/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:562239/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:252899/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:2756066/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:584641/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:256883/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:1411974/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:1188622/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:1426794/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:1676717/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:1704810/1‑68 (MQ=255) aagaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTAcggcg > 1:2123429/1‑68 (MQ=255) agaaagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGa < 1:1321550/69‑1 (MQ=255) aagGTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:959326/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:2311777/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:945022/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:772534/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:645805/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:1300879/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:3050821/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:297638/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:2835373/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCGAGCGAGAAACGTACGGCGGatat < 1:1977492/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:788115/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1894777/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1221983/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:3324681/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:304023/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2909895/69‑1 (MQ=255) aagCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1730485/69‑1 (MQ=255) gAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTccgcc < 1:3224747/69‑1 (MQ=255) gAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTccgcc < 1:1234928/69‑1 (MQ=255) tATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2593016/57‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTTGGTTCAAGCGAGAAACGTACGGCGGatat < 1:3120278/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:44769/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2277537/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2474841/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:695195/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1915197/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2790651/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1565057/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:422528/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:3419157/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2814043/53‑1 (MQ=255) cGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2092310/53‑1 (MQ=255) aaTCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTcc > 1:2001025/1‑54 (MQ=255) aaTCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTccc > 1:632346/1‑69 (MQ=255) aaTCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTccc > 1:1123033/1‑69 (MQ=255) aTCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:2151234/47‑1 (MQ=255) gCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGatat < 1:1396149/42‑1 (MQ=255) gAGCTGGGTTCAAGCGAGAACCGTACGGCGGatat < 1:3195445/35‑1 (MQ=255) aGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAAtt < 1:2291806/69‑1 (MQ=255) aGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAAtt < 1:2579924/69‑1 (MQ=255) ttCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAATTTATACa < 1:1853015/68‑1 (MQ=255) ttCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAATTTATACa < 1:334970/68‑1 (MQ=255) tCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAATTTATACa < 1:2426766/67‑1 (MQ=255) | CCGCAAGACAACGGAAAAGAAAGCTGAATGAGTATTCGCATAATCCCGCAAGATGAGCTGGGTTCAAGCGAGAAACGTACGGCGGATATGATTCCGCCGTTATTGTTCCCTCGGCTCAAGAATTTATACA > minE/2337119‑2337248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |