| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,340,553 | C→A | 30.2% | intergenic (‑347/‑26) | bipA ← / → glnA | GTP‑binding protein/glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,340,553 | 0 | C | A | 30.2% | 167.7 / 71.2 | 122 | intergenic (‑347/‑26) | bipA/glnA | GTP‑binding protein/glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base C (41/44); new base A (28/9); total (69/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGATGCTGAACGAGCACGAA > minE/2340487‑2340620 | gATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCAt < 1:517710/69‑1 (MQ=255)gATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCAt < 1:1071769/69‑1 (MQ=255)gATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCAt < 1:1801486/69‑1 (MQ=255)gATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCAt < 1:3337310/69‑1 (MQ=255) aTTTCGCTTTATCTTTTTTACGGCGACACGGCCCATTTAATTGCAGATTTCGTTACCACGACGACAATg < 1:1173660/69‑1 (MQ=255) aTTTCGCTTTATCTTTTTTACGGCGACACGGCCCATTTAATTGCAGATTTCGTTACCACGACGACAATg < 1:1380177/69‑1 (MQ=255) aTTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCAt < 1:1877903/68‑1 (MQ=255) tttCGCTTTATCTTTTTTACGGCGACACGGCCCATTTAATTGCAGATTTCTTTACCACGACGACAATg < 1:138186/68‑1 (MQ=255) tttCGCTTTATCTTTTTTACGGCGACACGGCCCATTTAATTGCAGATTTCGTTACCACGACGACAATg < 1:2402758/68‑1 (MQ=255) tttCGCTTTATCTTTTTTACGGCGACACGGCCCATTTAATTGCAGATTTCGTTACCACGACGACAATg < 1:844916/68‑1 (MQ=255) ttttttACGGCGACACGGCCCATTTAATTGCAGATTTCGTTACCACGACGACAATg < 1:2204347/56‑1 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:3117623/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:1708183/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:488289/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:3310152/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:1802936/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:2151810/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:2153028/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:2415737/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:2650008/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:772230/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:756023/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGgaga > 1:616499/1‑68 (MQ=255) ttttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACAAATCCAGgaga > 1:1176086/1‑68 (MQ=255) ttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGt < 1:1692140/68‑1 (MQ=255) ttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGt < 1:979767/68‑1 (MQ=255) ttACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGt < 1:2927042/68‑1 (MQ=255) aCGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTa < 1:375481/68‑1 (MQ=255) aCGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTa < 1:2753327/68‑1 (MQ=255) aCGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTa < 1:1942649/68‑1 (MQ=255) aCGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTa < 1:2871711/68‑1 (MQ=255) cGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTa < 1:793094/67‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:3413095/68‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:3352824/68‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:950291/68‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:1473088/68‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:225674/68‑1 (MQ=255) gCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:1475405/68‑1 (MQ=255) gCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCg > 1:2851350/1‑68 (MQ=255) ccAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGc > 1:1050311/1‑68 (MQ=255) cAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTg < 1:1482024/69‑1 (MQ=255) cAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTg < 1:2758957/69‑1 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2977537/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:301170/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2834489/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:93770/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:900511/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2807957/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:3089114/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:58602/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2643876/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1926043/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1121177/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1211367/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1901530/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2543417/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2400948/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1737118/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2315663/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1309979/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:1360557/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGgag > 1:2237942/2‑46 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACCAATCCAGga > 1:778046/2‑45 (MQ=255) ttaatTGCAGATTTCGTTACCACGACGACAATGACAAATCCAGgag > 1:1267955/2‑46 (MQ=255) ataatTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAaca > 1:2391160/1‑69 (MQ=255) ataatTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAaca < 1:1018226/69‑1 (MQ=255) aatTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACg < 1:487385/69‑1 (MQ=255) aatTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACg < 1:803441/69‑1 (MQ=255) atTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTa < 1:510749/53‑1 (MQ=255) atTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTa < 1:2674958/53‑1 (MQ=255) atTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTa < 1:3176680/53‑1 (MQ=255) tGCAGATTTCGTTATCACGACGACCATGACCAATCCAGGAGAGTTaaat < 1:592100/49‑2 (MQ=255) tGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTa < 1:324262/68‑1 (MQ=255) tGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTa < 1:3132625/68‑1 (MQ=255) tGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTa < 1:2844827/68‑1 (MQ=255) tGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTa < 1:1447096/68‑1 (MQ=255) gATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGAc < 1:1805039/69‑1 (MQ=255) ttCGTTCCCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGa > 1:436841/1‑68 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGa > 1:1017698/1‑68 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGa > 1:1193396/1‑68 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:1354182/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:3257101/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:3275865/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:259301/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:1222841/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:2588934/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:2774246/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:345221/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:745050/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:677480/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:231509/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:1891656/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:1883469/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:1656550/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:2167461/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGAt > 1:194990/1‑69 (MQ=255) ttCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACGGACGAt > 1:3344784/1‑69 (MQ=255) ccACGACGACCATGACCAATCCAGGAGAGTTAAAg < 1:2103171/35‑1 (MQ=255) cATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGATGCTGAACGAGCACgaa > 1:2899222/1‑68 (MQ=255) cATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGATGCTGAACGAGCACgaa > 1:2686807/1‑68 (MQ=255) | GATTTCGCTTTATCTTTTTTACGGCGACACGGCCAAAATAATTGCAGATTTCGTTACCACGACGACCATGACCAATCCAGGAGAGTTAAAGTATGTCCGCTGAACACGTACTGACGATGCTGAACGAGCACGAA > minE/2340487‑2340620 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |