BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,340,899	C→T	49.6%	S107S (TCC→TCT)	glnA →	glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,340,899	0	C	T	49.6%	‑0.6 / 108.1	117	S107S (TCC→TCT)	glnA	glutamine synthetase
Reads supporting (aligned to +/- strand): ref base C (32/27); new base T (18/40); total (50/67)
Fisher's exact test for biased strand distribution p-value = 1.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTACTGTTCGGGCCAG  >  minE/2340834‑2340966

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCATGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:1625157/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:2966393/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:1838347/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:1740038/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:1655413/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:2170317/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:2970283/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:843297/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:891522/68‑1 (MQ=255)

cTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTAt                                                                   <  1:2888327/68‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGGAAGGGTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:347281/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:2904479/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:1073906/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:2440404/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:290115/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:2494255/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:305024/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:3254545/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:2519013/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:1183848/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:887768/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:1427610/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:151593/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:1637079/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:422199/67‑1 (MQ=255)

     tATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:3419953/67‑1 (MQ=255)

     tATCCGTTGCGACACCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:1662719/67‑1 (MQ=255)

      aTCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCg                                                               <  1:2842900/66‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:657891/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:2754746/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:2981423/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:3074613/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:318576/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:3214313/68‑1 (MQ=255)

               gACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:3251355/68‑1 (MQ=255)

                aCATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCTATTGCGAAGCGCGCTGa                                                    <  1:3015118/67‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:2920231/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:982246/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:1150961/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:1843035/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:1315912/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:1596427/69‑1 (MQ=255)

                   tCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGAtt                                               <  1:246955/69‑1 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTa                                              >  1:978717/1‑68 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:2960264/1‑69 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:341092/1‑69 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:591299/1‑69 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:2293123/1‑69 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:2625455/1‑69 (MQ=255)

                     cTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTAc                                             >  1:774248/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCg                                                               >  1:3328011/1‑49 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAATCGCGCCGAAGATTACCt                                           >  1:1430590/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:2798026/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:3321859/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:406900/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:408274/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:2539224/1‑69 (MQ=255)

                       tGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:657681/1‑69 (MQ=255)

                       agAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCt                                           >  1:3110754/2‑69 (MQ=255)

                              ggCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCTAAGCGCGCCGAAGATTACCTGCGTTcc                                    >  1:3096909/1‑69 (MQ=255)

                              ggCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTcc                                    >  1:547364/1‑69 (MQ=255)

                                   cccgCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:3122889/66‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGTGCCGAAGATTACCTGCGTTCCACTgg                               <  1:2572860/69‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:475994/69‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:2822651/69‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:2041993/69‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:2295629/69‑1 (MQ=255)

                                   ccTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTgg                               <  1:871577/69‑1 (MQ=255)

                                    cTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGc                              <  1:431505/69‑1 (MQ=255)

                                    cTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGc                              <  1:984693/69‑1 (MQ=255)

                                    cTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGc                              <  1:2600892/69‑1 (MQ=255)

                                    cTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGc                              <  1:2279509/69‑1 (MQ=255)

                                     tGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGc                              <  1:1649012/68‑1 (MQ=255)

                                          ggCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGGCGAAGATTACCTGCGTTCCACTGGCATTGcc                        <  1:1897646/69‑1 (MQ=255)

                                                 aCCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCg                 >  1:184145/1‑69 (MQ=255)

                                                 aCCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCg                 >  1:1970355/1‑69 (MQ=255)

                                                 aCCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCg                 <  1:3167388/69‑1 (MQ=255)

                                                 aCCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCg                 >  1:2043776/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGt                >  1:2739189/1‑68 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2614446/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:3229916/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2722643/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2828399/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2861652/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:1573115/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2877725/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:521415/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:488897/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2125066/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:2999739/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCTATTGCGAAGCGCGCTGAAGATTACCTGCGTTCTACTGGCATTGCCGACACCGTa               >  1:1723436/1‑69 (MQ=255)

                                                   cGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGt                <  1:382544/68‑1 (MQ=255)

                                                   cGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGt                <  1:939413/68‑1 (MQ=255)

                                                    gTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTAc              >  1:2242233/1‑69 (MQ=255)

                                                    gTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTAc              >  1:983561/1‑69 (MQ=255)

                                                       aCCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGcc                        >  1:3099144/1‑56 (MQ=255)

                                                       aCCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGcc                        >  1:1245442/1‑56 (MQ=255)

                                                       aCCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGcc                        >  1:1227555/1‑56 (MQ=255)

                                                                ccATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTACTGTTCGGGCCAg  >  1:2016981/1‑69 (MQ=255)

                                                                ccATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTACTGTTCGGGCCAg  >  1:351061/1‑69 (MQ=255)

CTGATTATCCGTTGCGACATCCTTGAACCTGGCACCCTGCAAGGCTATGACCGTGACCCGCGCTCCATTGCGAAGCGCGCCGAAGATTACCTGCGTTCCACTGGCATTGCCGACACCGTACTGTTCGGGCCAG  >  minE/2340834‑2340966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`