| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,342,351 | C→T | 27.5% | N26N (AAC→AAT) | glnL → | sensory kinase in two‑component regulatory system with GlnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,342,351 | 0 | C | T | 27.5% | 106.1 / 36.9 | 94 | N26N (AAC→AAT) | glnL | sensory kinase in two‑component regulatory system with GlnG |
| Reads supporting (aligned to +/- strand): ref base C (56/12); new base T (8/18); total (64/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTCC > minE/2342292‑2342405 | cccGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAATCTGGCGATc > 1:1012041/1‑69 (MQ=255)cccGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAATCTGGCGATc > 1:2872828/1‑69 (MQ=255) tGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTa < 1:742417/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:581096/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:3067895/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:2505721/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:2350512/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:2336222/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:2063917/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:52654/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:1345931/69‑1 (MQ=255) gggCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGcc < 1:971082/69‑1 (MQ=255) cAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAAc < 1:185090/69‑1 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCaa > 1:3215133/1‑64 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:848697/1‑68 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:693041/1‑68 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:1681656/1‑68 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:1751792/1‑68 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:2524199/1‑68 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:3376683/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:1149286/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:2002602/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:3167323/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:626732/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:303227/1‑69 (MQ=255) tCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:1306414/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCt > 1:1214559/1‑67 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTg > 1:840596/1‑68 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:2926176/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:2363795/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:334793/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:285443/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:150489/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:637912/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:1295397/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:586348/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:550472/1‑69 (MQ=255) ccTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGc > 1:1996258/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:922789/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:733951/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:1086488/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:2351438/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:2324716/1‑69 (MQ=255) aTTAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCATTACGCCAACCCCGCCGCGCAACAACTg > 1:3395240/1‑69 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:103786/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:3123980/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:929825/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:416232/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:646507/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1548591/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2045277/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2037087/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2089114/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1780985/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1693941/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2226212/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1608255/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2377954/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2506720/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1231801/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:1082531/40‑1 (MQ=255) tAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCt > 1:2618645/1‑69 (MQ=255) tAACAGTATTTTGTGAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCt > 1:963604/1‑69 (MQ=255) aaCAGTATTTTGTTAATCGATGACAATCTGGCGATCCAt < 1:2658269/39‑1 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:82377/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:2690103/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:1523413/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:1483403/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:90974/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:1984646/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:57649/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:2051397/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:393317/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:2645483/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:3374273/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:477866/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:2595294/1‑61 (MQ=255) aaCAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGcaac > 1:2754151/1‑61 (MQ=255) tATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGcc > 1:2223602/1‑67 (MQ=255) ttttGTTAATCGATGACAACCTGGCGATCCATTACGCCAAc < 1:180590/41‑1 (MQ=255) ttttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCaaa > 1:3406935/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCaaa > 1:903116/1‑67 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:2784371/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:860157/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:2891532/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:80888/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:2687852/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:2401743/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:1873100/1‑69 (MQ=255) ttGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGc > 1:582710/1‑69 (MQ=255) tAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTcc > 1:106772/1‑68 (MQ=255) tAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTcc > 1:2535846/1‑68 (MQ=255) tAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTcc > 1:1530242/1‑68 (MQ=255) tAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTcc > 1:3261296/1‑68 (MQ=255) | CCCGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCTGGCGATCCATTACGCCAACCCTGCCGCGCAACAACTGCTCGCCCAAAGCTCC > minE/2342292‑2342405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |