| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,342,948 | T→C | 33.3% | D225D (GAT→GAC) | glnL → | sensory kinase in two‑component regulatory system with GlnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,342,948 | 0 | T | C | 33.3% | 40.8 / 29.1 | 57 | D225D (GAT→GAC) | glnL | sensory kinase in two‑component regulatory system with GlnG |
| Reads supporting (aligned to +/- strand): ref base T (15/23); new base C (7/12); total (22/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAATATTGTG > minE/2342895‑2343011 | aCGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGaa > 1:2698530/1‑69 (MQ=255)aCGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGaa > 1:2270199/1‑69 (MQ=255)aCGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGaa > 1:1795378/1‑69 (MQ=255)aCGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGaa > 1:2274089/1‑69 (MQ=255) gATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGa < 1:291740/69‑1 (MQ=255) gATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGa > 1:1284414/1‑69 (MQ=255) gATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGa < 1:2782090/69‑1 (MQ=255) gATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGa > 1:578797/1‑69 (MQ=255) aTGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGa < 1:2379125/68‑1 (MQ=255) gAAGTGCCGGACAACGTGCGGTTGATTCGTGATTAAGATCCCAGCCTACCGGa > 1:587286/1‑53 (MQ=255) gAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGa > 1:2046016/1‑53 (MQ=255) gAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGa > 1:2924895/1‑53 (MQ=255) gAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGa > 1:725157/1‑53 (MQ=255) aCTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGa < 1:400153/51‑1 (MQ=255) gCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCa < 1:1679110/69‑1 (MQ=255) gCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCa < 1:638745/69‑1 (MQ=255) gCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCa < 1:150300/69‑1 (MQ=255) gCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCa < 1:2862467/69‑1 (MQ=255) aCAACGTGCGGTTGATTCGTGATTACGATCCCCGCCTACCGGAACTGGCGCACGACCCCGATCAAATTg < 1:1173817/69‑1 (MQ=255) aCAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTg < 1:2877280/69‑1 (MQ=255) aCAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTg < 1:3303258/69‑1 (MQ=255) aCAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTg < 1:3234438/69‑1 (MQ=255) aCAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTg < 1:540998/69‑1 (MQ=255) aaCGTGCGGTT‑ATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAAc < 1:1518654/69‑1 (MQ=255) aCGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAAc < 1:1865905/69‑1 (MQ=255) aCGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAAc < 1:588301/69‑1 (MQ=255) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATc > 1:203314/1‑50 (MQ=37) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:951633/1‑68 (MQ=39) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:711355/1‑68 (MQ=39) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:2680112/1‑68 (MQ=39) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:1789679/1‑68 (MQ=39) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:2601149/1‑68 (MQ=39) gATTCGGGATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGc > 1:1927026/1‑68 (MQ=39) cGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGaa > 1:1374954/1‑68 (MQ=255) cGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCAGGATCAAATTGAACAGGTCTTGcag > 1:1163404/1‑64 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:2253093/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:2092741/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:2788822/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:3006337/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:1613702/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:1662084/68‑1 (MQ=255) tGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAata < 1:50291/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:1577098/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:167477/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:1421446/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:1800124/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:1779150/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:42925/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:1904491/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:3136979/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:219469/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:2773135/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:2488490/68‑1 (MQ=255) gATTACGACCCCAGCCTGCCGGAACTGGAGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAatat < 1:827836/68‑1 (MQ=255) aCGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAATATtgtg > 1:541576/1‑68 (MQ=255) aCGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAATATtgtg > 1:3286091/1‑68 (MQ=255) aCGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAATATtgtg > 1:2388473/1‑68 (MQ=255) | ACGCTGGTGTCGATGGAACTGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTACCGGAACTGGCGCACGACCCGGATCAAATTGAACAGGTCTTGCTGAATATTGTG > minE/2342895‑2343011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |