| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,343,955 | A→C | 21.5% | S207S (TCA→TCC) | glnG → | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,343,955 | 0 | A | C | 21.5% | 130.3 / 33.9 | 79 | S207S (TCA→TCC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base A (24/38); new base C (15/2); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACA > minE/2343888‑2344023 | gTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAg > 1:2550343/1‑69 (MQ=255)gTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAg > 1:1871613/1‑69 (MQ=255)gTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAg > 1:3200797/1‑69 (MQ=255)gTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAg > 1:2191544/1‑69 (MQ=255)gTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAg > 1:2840404/1‑69 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:133782/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:1390743/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:472172/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:1187467/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:30027/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:3045352/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGa < 1:896196/67‑1 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAAc > 1:1344122/1‑69 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAAc > 1:869382/1‑69 (MQ=255) cgcgcgCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAAc > 1:3292539/1‑69 (MQ=255) cgcgcCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTg < 1:519497/69‑1 (MQ=255) cgcCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGcc > 1:411041/1‑66 (MQ=255) cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga < 1:2655344/69‑1 (MQ=255) cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga < 1:2839195/69‑1 (MQ=255) cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga < 1:994434/69‑1 (MQ=255) cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga < 1:3336976/69‑1 (MQ=255) tATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAgg > 1:416172/1‑69 (MQ=255) tATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAgg > 1:2466493/1‑69 (MQ=255) aTCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGgc > 1:2893396/1‑69 (MQ=255) aTCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGgc > 1:37460/1‑69 (MQ=255) gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt < 1:2125558/69‑1 (MQ=255) gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt < 1:2133681/69‑1 (MQ=255) gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt < 1:852066/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:3273378/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:3407160/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:29761/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:653244/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:2774305/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:1157286/69‑1 (MQ=255) gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg < 1:2340768/69‑1 (MQ=255) tATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATAc < 1:2514510/69‑1 (MQ=255) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1031823/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1551141/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1741576/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1781850/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1827942/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:3076302/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:1957089/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2982820/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2896671/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2289714/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2623740/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2408691/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:2326230/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc > 1:27543/1‑69 (MQ=25) aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAc > 1:2671945/1‑68 (MQ=25) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:118690/1‑69 (MQ=255) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:386046/1‑69 (MQ=255) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:3347594/1‑69 (MQ=255) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:647299/1‑69 (MQ=255) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:2798457/1‑69 (MQ=255) ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt > 1:2963908/1‑69 (MQ=255) tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg > 1:679080/1‑69 (MQ=255) tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg > 1:955934/1‑69 (MQ=255) tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg > 1:208336/1‑69 (MQ=255) attGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg > 1:942335/2‑69 (MQ=255) tcgATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATACCAt < 1:1782663/57‑1 (MQ=25) tGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATACCAt < 1:2146601/58‑1 (MQ=25) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1766870/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1823854/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:631143/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1322134/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1199424/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:2214205/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:3314843/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1843907/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1869235/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:3211704/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1940411/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:3115739/68‑1 (MQ=255) aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:1967097/68‑1 (MQ=255) aTCAGAACTGTATGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:464777/68‑1 (MQ=255) tCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg < 1:2687079/67‑1 (MQ=255) cAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAAc > 1:2478355/1‑69 (MQ=255) aGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACa > 1:1796866/1‑69 (MQ=255) | GTCCGCGCGCCAAAGCGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACA > minE/2343888‑2344023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |