Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,343,967 C→T 26.8% G211G (GGC→GGT glnG → fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,343,9670CT26.8% 71.9 / 22.6 63G211G (GGC→GGTglnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)
Reads supporting (aligned to +/- strand):  ref base C (16/30);  new base T (15/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACA  >  minE/2343903‑2344023
                                                                |                                                        
cgcCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGcc                                                         >  1:411041/1‑66 (MQ=255)
   cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga                                                   <  1:994434/69‑1 (MQ=255)
   cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga                                                   <  1:2655344/69‑1 (MQ=255)
   cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga                                                   <  1:3336976/69‑1 (MQ=255)
   cGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACgaga                                                   <  1:2839195/69‑1 (MQ=255)
       tATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAgg                                               >  1:2466493/1‑69 (MQ=255)
       tATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAgg                                               >  1:416172/1‑69 (MQ=255)
        aTCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGgc                                              >  1:37460/1‑69 (MQ=255)
        aTCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGgc                                              >  1:2893396/1‑69 (MQ=255)
             gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt                                         <  1:2125558/69‑1 (MQ=255)
             gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt                                         <  1:2133681/69‑1 (MQ=255)
             gcTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGtt                                         <  1:852066/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:29761/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:3273378/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:2774305/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:3407160/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:653244/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:2340768/69‑1 (MQ=255)
                       gCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGcgcg                               <  1:1157286/69‑1 (MQ=255)
                            tATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATAc                          <  1:2514510/69‑1 (MQ=255)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2623740/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2982820/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2896671/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1827942/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:3076302/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:27543/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1551141/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1781850/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2408691/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1957089/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2326230/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:2289714/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1031823/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAcc                         >  1:1741576/1‑69 (MQ=25)
                             aTCCCGAAGGATTTGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATAc                          >  1:2671945/1‑68 (MQ=25)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:386046/1‑69 (MQ=255)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:118690/1‑69 (MQ=255)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:2798457/1‑69 (MQ=255)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:647299/1‑69 (MQ=255)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:3347594/1‑69 (MQ=255)
                                ccAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCAtt                      >  1:2963908/1‑69 (MQ=255)
                                        tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg              >  1:208336/1‑69 (MQ=255)
                                        tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg              >  1:679080/1‑69 (MQ=255)
                                        tttGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg              >  1:955934/1‑69 (MQ=255)
                                        attGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAggg              >  1:942335/2‑69 (MQ=255)
                                         tcgATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATACCAt                       <  1:1782663/57‑1 (MQ=25)
                                          tGATCGAATCCGAACTGTTTGGTCACGAGAAAGGCGCATTTACCGGCGCGAATACCAt                       <  1:2146601/58‑1 (MQ=25)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:631143/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1199424/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1322134/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1967097/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1766870/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:3314843/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:3211704/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:3115739/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1823854/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1843907/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1869235/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:1940411/68‑1 (MQ=255)
                                                 aTCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:2214205/68‑1 (MQ=255)
                                                 aTCAGAACTGTATGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:464777/68‑1 (MQ=255)
                                                  tCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTg      <  1:2687079/67‑1 (MQ=255)
                                                   cAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAAc   >  1:2478355/1‑69 (MQ=255)
                                                    aGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACa  >  1:1796866/1‑69 (MQ=255)
                                                                |                                                        
CGCCGTTTATCGCGCTGAATATGGCAGCTATCCCAAAAGATTTGATCGAATCAGAACTGTTTGGCCACGAGAAAGGCGCGTTTACTGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACA  >  minE/2343903‑2344023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: