| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,344,243 | T→C | 27.6% | V303V (GTT→GTC) | glnG → | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,344,243 | 0 | T | C | 27.6% | 86.3 / 34.3 | 83 | V303V (GTT→GTC) | glnG | fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI) |
| Reads supporting (aligned to +/- strand): ref base T (26/34); new base C (20/3); total (46/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTT > minE/2344175‑2344310 | cACCAGAATCTCGAACGGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:579467/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1357338/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1779072/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1940778/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3136342/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3072374/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:3021106/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:754533/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2556845/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2303021/69‑1 (MQ=255)cACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:2385674/69‑1 (MQ=255) tccAGAATCTCGAACAGCGAGTGCACGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGtt < 1:1604848/67‑1 (MQ=255) cAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTAt > 1:1155683/1‑68 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2708352/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2094469/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1847375/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:296839/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2279506/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:2469874/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:3355626/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:763080/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1700842/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATcc > 1:1463296/1‑69 (MQ=255) aGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACAGCCTGAACGTTATcc > 1:1308780/1‑69 (MQ=255) tcGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc > 1:2016032/1‑69 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATGCGCGTTc < 1:1502828/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1225060/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2131185/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2107789/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2757693/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2762000/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:707830/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1983062/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1549420/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1134264/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1837680/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:1797259/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:2312601/65‑1 (MQ=255) aCAGCGAGTGCAGGAAGGTAAGTTCAGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTc < 1:258240/65‑1 (MQ=255) aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:964967/69‑1 (MQ=255) aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:2302967/69‑1 (MQ=255) aaGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg < 1:1061581/69‑1 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2564445/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:923219/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:249932/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:586705/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3234180/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1077966/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:394107/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3372000/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2294854/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1782561/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2279807/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1896981/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1963023/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:1162794/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:214668/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:2031105/1‑51 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTgccg > 1:1531049/1‑50 (MQ=255) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCATACGCGTTCATCTgccgc > 1:1964474/1‑51 (MQ=38) tCCGTGAGGATCTGTTCCACCGCCTGAACGTCAACCGCGt > 1:422028/1‑40 (MQ=37) tCCGTGAGGATCTGTTACACCGCCTGAACGTCATCCGCGTTCATCTgccgc > 1:3425765/1‑51 (MQ=255) tGAGGATCTGTTCCACCGCGTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:1644895/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:840826/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:93331/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:1720093/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:2030219/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:148186/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:1246293/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:2235056/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:3323845/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAg < 1:3328359/68‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2435943/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:726482/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2484888/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:3217208/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:27375/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:809444/69‑1 (MQ=255) tGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGa < 1:2767793/69‑1 (MQ=255) aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:2179620/69‑1 (MQ=255) aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:666079/69‑1 (MQ=255) aTCTGTTCCACCGCCTGAACGTCATCCGCGTTCATCTGCCGCCACTGCGCGAACGTCGGGAAGATATTc < 1:785700/69‑1 (MQ=255) tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATAttac > 1:3316893/1‑67 (MQ=255) tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:991048/1‑69 (MQ=255) tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:2875004/1‑69 (MQ=255) tCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTcc > 1:2168429/1‑69 (MQ=255) tCTGTTCAACCGCCTGAACGTTATCCGCGTTCATCTgccgcc > 1:932874/1‑42 (MQ=255) gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGcgcg > 1:2465199/1‑39 (MQ=255) gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:3378410/1‑69 (MQ=255) gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:3183695/1‑69 (MQ=255) gCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcgc > 1:2800538/1‑69 (MQ=255) aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGatat > 1:129670/1‑50 (MQ=255) aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGgcg > 1:2070338/1‑63 (MQ=255) aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAt > 1:1744494/1‑69 (MQ=255) aaCGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAt > 1:2104267/1‑69 (MQ=255) ttATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAttttt < 1:416901/69‑1 (MQ=255) ttATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCAttttt < 1:1172298/69‑1 (MQ=255) | CACCAGAATCTCGAACAGCGAGTGCAGGAAGGTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGCGAACGTCGGGAAGATATTCCCCGTCTGGCGCGCCATTTTT > minE/2344175‑2344310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |