Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,344,646 T→C 70.5% L438L (TTG→CTG)  glnG → fused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,344,6460TC70.5% 70.5 / 30.2 71L438L (TTG→CTG) glnGfused DNA‑binding response regulator in two‑component regulatory system with GlnL, nitrogen regulator I (NRI)
Reads supporting (aligned to +/- strand):  ref base T (4/17);  new base C (22/28);  total (26/45)
Fisher's exact test for biased strand distribution p-value = 6.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCT  >  minE/2344581‑2344710
                                                                 |                                                                
ccGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGc                                                               >  1:2200729/1‑69 (MQ=255)
     catcaAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACAt                                                          >  1:1560341/1‑69 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCTACATACGCAg                                                    <  1:1630692/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2748172/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1858035/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1869967/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:22032/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:231259/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2318412/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2338347/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1008579/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2354376/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2885643/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2973585/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:3140000/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:3317342/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:367430/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:367765/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:380155/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:925064/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:969726/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1346274/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1134435/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1668475/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1421529/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1459121/66‑1 (MQ=255)
           aaccTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:115513/66‑1 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:1787032/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2579592/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2714367/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2718234/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2814253/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:552629/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:3010608/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:1227469/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:3192603/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:3201138/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:323963/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2401722/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:67402/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:721587/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:73675/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:173355/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2078155/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:909930/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:2004081/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:110384/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:943905/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGc                                                               >  1:1800669/3‑57 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:1613791/66‑1 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:49390/66‑1 (MQ=255)
            accTGCTTTCCGAAGCACAGCCAGAGCTGGAACGGACGTTACTGACCACCGCGCTGCGACATACGCAg                                                    <  1:2237479/66‑1 (MQ=255)
                         aGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGa                                      <  1:2898563/69‑1 (MQ=255)
                         aGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGa                                      <  1:836215/69‑1 (MQ=255)
                         aGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGa                                      <  1:276397/69‑1 (MQ=255)
                          gcgcAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGa                                      <  1:2559925/68‑1 (MQ=255)
                                      cTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTa                         <  1:3371763/69‑1 (MQ=255)
                                      cTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTa                         <  1:1756436/69‑1 (MQ=255)
                                      cTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTa                         <  1:202313/69‑1 (MQ=255)
                                                cGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTggg               >  1:3060421/1‑69 (MQ=255)
                                                cGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTggg               >  1:2185969/1‑69 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1649376/69‑1 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1783861/69‑1 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1694698/69‑1 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1544615/69‑1 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:478431/69‑1 (MQ=255)
                                                             cgcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:284881/69‑1 (MQ=255)
                                                              gcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:694871/68‑1 (MQ=255)
                                                              gcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1725343/68‑1 (MQ=255)
                                                              gcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1388841/68‑1 (MQ=255)
                                                              gcgTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCt  <  1:1083277/68‑1 (MQ=255)
                                                                 |                                                                
CCGGTCATCAAAATCTGCTTTCCGAAGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAAGCGGCGCGGCTACTCGGCTGGGGCCGCAACACCCT  >  minE/2344581‑2344710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: