| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,353,113 | A→C | 17.8% | F434L (TTT→TTG) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,353,113 | 0 | A | C | 17.8% | 181.6 / 34.6 | 101 | F434L (TTT→TTG) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (27/56); new base C (8/10); total (35/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
AAACAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCGCA > minE/2353046‑2353179 | aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:2639475/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:956085/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:874444/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:769031/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:3322241/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:2836765/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:2801141/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:24572/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:1671696/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:1584856/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:1395419/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGa < 1:1039083/68‑1 (MQ=255)aaaCAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGATGTCAATCGTGATTAACTGa < 1:3435225/68‑1 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:1331800/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:2623888/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:3319074/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:2539628/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:353050/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:1087545/1‑69 (MQ=255) acaacTTTACCCGGTGTATAACGCAGTTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAAc > 1:586501/1‑69 (MQ=255) acaacTTTACCCGGTGTAAAACGCAG‑TTCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGCAACa > 1:3082889/1‑69 (MQ=255) tttACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTg < 1:2342946/69‑1 (MQ=255) ccGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCt < 1:3033139/68‑1 (MQ=255) ccGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCt < 1:1651095/68‑1 (MQ=255) ccGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCt < 1:543363/68‑1 (MQ=255) ggTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAAt < 1:2286237/69‑1 (MQ=255) ggTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAAt < 1:2131906/69‑1 (MQ=255) tgtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTg < 1:1468599/60‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:1810677/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:1958004/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:1779831/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:2898168/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:115316/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:281840/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:2223495/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:383659/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:2475134/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTaa < 1:1604517/65‑1 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtc > 1:2984165/1‑69 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtc > 1:2381403/1‑69 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtc > 1:2361634/1‑69 (MQ=255) gtATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGt > 1:1646045/1‑68 (MQ=255) taACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat < 1:232473/68‑1 (MQ=255) taACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat < 1:2181200/68‑1 (MQ=255) taACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat < 1:1347987/68‑1 (MQ=255) taACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat < 1:2733752/68‑1 (MQ=255) aCGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat > 1:572329/1‑66 (MQ=255) aCGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat > 1:3089474/1‑66 (MQ=255) aCGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGtcat > 1:910955/1‑66 (MQ=255) aCGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTcatcat > 1:1008086/1‑69 (MQ=255) aCGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTcatcat > 1:1067174/1‑69 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAAGTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:523856/69‑1 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:2721234/69‑1 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:1275035/69‑1 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:802862/69‑1 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:2087723/69‑1 (MQ=255) cGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTg < 1:2911029/69‑1 (MQ=255) aGGCTGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:252966/69‑1 (MQ=255) aGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:1190224/69‑1 (MQ=255) aGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:3069673/69‑1 (MQ=255) aGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:901054/69‑1 (MQ=255) ggCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGttt < 1:661100/69‑1 (MQ=255) ggCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGttt < 1:1739094/69‑1 (MQ=255) ggCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGttt < 1:1493328/69‑1 (MQ=255) aTTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGc > 1:1221455/1‑69 (MQ=255) aTTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGc > 1:2603352/1‑69 (MQ=255) aTTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGc > 1:1992609/1‑69 (MQ=255) gttgtCTATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTg > 1:441756/1‑69 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTaa > 1:2713190/1‑36 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTaa > 1:747480/1‑36 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:2980799/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:541541/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:328692/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:1267005/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:2759567/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:3159886/59‑1 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTg > 1:1116874/1‑69 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTg > 1:3396769/1‑69 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTg > 1:3135151/1‑69 (MQ=255) gttgtCAATCGTGATTAACTGAAACATCTGACCTAATGTAATCATCCCGCTGATTTGtt < 1:1204933/59‑1 (MQ=255) gttgtCAATCCTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGtt < 1:88691/59‑1 (MQ=255) cGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTgcgc > 1:1607441/1‑69 (MQ=255) cGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTgcgc > 1:2627386/1‑69 (MQ=255) cGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTgcgc > 1:309248/1‑69 (MQ=255) cGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTgcgc > 1:2565101/1‑69 (MQ=255) aaCTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTc > 1:3285482/1‑68 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTTTTTATCAGCCCGCTTTTGCGCttttt < 1:3026796/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:1265111/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:731085/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:1292313/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:494336/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:3424626/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:2602919/65‑1 (MQ=255) cTGCAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCCGCTTTTGCGCttttt < 1:318541/65‑1 (MQ=255) cTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCg < 1:166311/68‑1 (MQ=255) cTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCg < 1:2240645/68‑1 (MQ=255) cTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCg < 1:808768/68‑1 (MQ=255) cTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCg < 1:340806/68‑1 (MQ=255) cTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCg < 1:3012229/68‑1 (MQ=255) gAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCGCa > 1:1549852/1‑68 (MQ=255) | AAACAACTTTACCCGGTGTATAACGCAGCTGCAGCGAGGCGGTATTGTTGTCAATCGTGATTAACTGAAACATCTGACCTAATGTCATCATCCCGCTGATTTGTTTATCAGCCTGCTTTTGCGCTTTTTCCGCA > minE/2353046‑2353179 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |