| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,353,879 | C→T | 19.2% | G179D (GGT→GAT) | yihF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,353,879 | 0 | C | T | 19.2% | 101.8 / 13.7 | 67 | G179D (GGT→GAT) | yihF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (35/19); new base T (10/3); total (45/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
GGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATACTCTGCAGGGGC > minE/2353816‑2353940 | ggTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATc > 1:3276745/1‑68 (MQ=255)ggTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATc > 1:1287216/1‑68 (MQ=255)ggTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATc > 1:2460206/1‑68 (MQ=255)ggTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATc > 1:2324525/1‑68 (MQ=255)ggTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATc > 1:2763871/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:2209233/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:1459763/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:149863/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:2862945/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:1988152/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:2716734/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:1808067/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:716749/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:728739/1‑68 (MQ=255) tGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTATCATCACCATTaaa > 1:1135725/1‑68 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:2111205/68‑1 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:2895000/68‑1 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:3434297/68‑1 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:3430557/68‑1 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:2266583/68‑1 (MQ=255) gAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:2489389/68‑1 (MQ=255) aCAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAAc > 1:1484175/1‑69 (MQ=255) aCAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAAc > 1:140490/1‑69 (MQ=255) aCAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAAc > 1:2548401/1‑69 (MQ=255) cAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACt > 1:1088276/1‑69 (MQ=255) cAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACt > 1:1722637/1‑69 (MQ=255) tCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa < 1:523928/60‑1 (MQ=255) ttCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTc > 1:1523450/1‑69 (MQ=255) tGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCaaaa > 1:2894656/1‑68 (MQ=255) tGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCaaaa > 1:1257553/1‑68 (MQ=255) tGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCaaaa > 1:569299/1‑68 (MQ=255) tGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCaaaa > 1:2408086/1‑68 (MQ=255) tGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCaaaa > 1:638780/1‑68 (MQ=255) tCAATATCCAGGTTAGACAATGAACTATCATCACCATTAAAAGTAAACTGGCCTTCACCAAAAGAAAAc < 1:1799489/69‑1 (MQ=255) tCAATATCCAGGTTAGACAATGAACTATCATCACCATTAAAAGTAAACTGGCCTTCACCAAAAGAAAAc < 1:2849461/69‑1 (MQ=255) tCAATATCCAGGTTAGACAATGAACTATCATCACCATTAAAAGTAAACTGGCCTTCACCAAAAGAAAAc < 1:1327696/69‑1 (MQ=255) tCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAAc < 1:1652279/69‑1 (MQ=255) tCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAAc < 1:3156604/69‑1 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGc > 1:30190/1‑47 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:961181/1‑68 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:43319/1‑68 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:2942704/1‑68 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:1134145/1‑68 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:237129/1‑68 (MQ=255) atCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc > 1:1719204/1‑68 (MQ=255) tCCAGTTTAGACAATGAACTACCATCACCATTAAAAGTa > 1:1395963/1‑39 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa > 1:2664669/1‑39 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa > 1:1318911/1‑39 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa > 1:481147/1‑39 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTa > 1:1610933/1‑39 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:115642/1‑65 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:3409306/1‑65 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACt > 1:1816481/1‑64 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTcc > 1:1397082/1‑68 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCa < 1:202638/69‑1 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCa < 1:2947564/69‑1 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCa < 1:646161/69‑1 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCa > 1:3252870/1‑69 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCa > 1:2799432/1‑69 (MQ=255) tCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTCGccc > 1:2973677/1‑48 (MQ=255) cAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATa < 1:520434/69‑1 (MQ=255) aaTGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCAt < 1:1320829/58‑1 (MQ=255) aaTGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCAt < 1:3296694/58‑1 (MQ=255) aTGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTc < 1:2273837/54‑1 (MQ=255) tGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATACTCTGCAGGGGc < 1:754495/69‑1 (MQ=255) gAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATACTCTGCAGGGGc < 1:3366989/68‑1 (MQ=255) gAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATACTCTGCAGGGGc < 1:1605849/68‑1 (MQ=255) ccATCACCATTAAAAGTAAAGTGGCCCTCACCAAAAGAAAACtt < 1:2390555/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt < 1:1396340/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:1392307/1‑44 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:3273506/1‑44 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt < 1:1370853/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt < 1:53952/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt < 1:1462253/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:3154791/1‑44 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:1236877/1‑44 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt < 1:933989/44‑1 (MQ=255) ccATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACtt > 1:264043/1‑44 (MQ=255) | GGTGATAATTGCAGAACAATATCTTCGACTTTGCCTTCAATATCCAGGTTAGACAATGAACTACCATCACCATTAAAAGTAAACTGGCCCTCACCAAAAGAAAACTTTCCATACTCTGCAGGGGC > minE/2353816‑2353940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |