| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,355,004 | A→G | 32.8% | N64N (AAT→AAC) | dsbA ← | periplasmic protein disulfide isomerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,355,004 | 0 | A | G | 32.8% | 104.7 / 71.9 | 132 | N64N (AAT→AAC) | dsbA | periplasmic protein disulfide isomerase I |
| Reads supporting (aligned to +/- strand): ref base A (35/53); new base G (7/36); total (42/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.06e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CACCACCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGAAA > minE/2354940‑2355064 | caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1880703/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:2892560/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1897659/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:1913320/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:2494500/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:864150/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACAtt > 1:943559/1‑67 (MQ=255)caccacCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTAACAtt > 1:2471693/1‑67 (MQ=255) gTTGACGTTGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:128768/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCa > 1:78522/1‑68 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:2552337/1‑69 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:3338475/1‑69 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAg > 1:233387/1‑69 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1460274/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1649440/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3391385/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1839665/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1842479/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1589400/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3333868/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:2397002/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3301894/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:1019712/69‑1 (MQ=255) gTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAg < 1:3059994/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACTTTATCAGAAATATGCAGAACttcttc < 1:1654369/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2186863/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1008339/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:558745/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2818165/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:2839914/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:501433/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:911244/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1485681/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1187207/69‑1 (MQ=255) gTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:408384/69‑1 (MQ=255) tATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:3023795/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:3211633/67‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1842470/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:312978/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:630554/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1963623/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1550829/68‑1 (MQ=255) aTTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa < 1:1324469/68‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:368026/1‑65 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:2907717/69‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:1059162/1‑69 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:1377342/69‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:3166756/69‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:2534015/1‑69 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:3386006/69‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:193459/69‑1 (MQ=255) tAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt > 1:2030234/1‑69 (MQ=255) aGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:340220/68‑1 (MQ=255) aGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACt < 1:1496972/68‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:537202/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:1626957/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:519911/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:1447454/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:2287927/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:3254870/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:273701/69‑1 (MQ=255) aTCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAg < 1:3387410/69‑1 (MQ=255) cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2169569/68‑1 (MQ=255) cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2395682/68‑1 (MQ=255) cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1334913/68‑1 (MQ=255) cTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2545771/68‑1 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATGAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:219449/1‑69 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:488971/1‑57 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:499455/1‑57 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:2894024/1‑57 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:2482710/1‑57 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCa > 1:694012/1‑57 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:2212786/1‑69 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:3356334/1‑69 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:139052/1‑69 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:45033/1‑69 (MQ=255) ttCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAg > 1:943684/1‑69 (MQ=255) cTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTGAAACTGATAGCAGTGCg > 1:686654/1‑66 (MQ=255) cTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCg > 1:2756128/1‑66 (MQ=255) cGGCAGTTTTTTCTTCACGTTATCAGAAATATGCAGAACttcttc < 1:1230644/45‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:657001/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:785771/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:910729/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:953957/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2945520/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2869843/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2768363/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:2600096/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1957222/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1624850/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1430858/48‑1 (MQ=255) ttttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:101273/48‑1 (MQ=255) tttCTTCACGTTATCAGAAATATGCAGAACTTCTTCAAACTGATAGc < 1:1403051/47‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:2951521/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1890630/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1863574/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1859678/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1762941/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1759056/68‑1 (MQ=255) tcttcACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGaaa < 1:1154492/68‑1 (MQ=255) | CACCACCCATGAAGTTGACGTGGTATTTAGTCATCTTCACGCCTTCCGGCAGTTTTTTCTTCACATTATCAGAAATATGCAGAACTTCTTCAAACTGATAGCAGTGCGGGCAGAAGAAAGAGAAA > minE/2354940‑2355064 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |