| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,356,630 | C→T | 22.3% | T6M (ACG→ATG) | mobA → | molybdopterin‑guanine dinucleotide synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,356,630 | 0 | C | T | 22.3% | 123.0 / 25.4 | 89 | T6M (ACG→ATG) | mobA | molybdopterin‑guanine dinucleotide synthase |
| Reads supporting (aligned to +/- strand): ref base C (30/39); new base T (13/7); total (43/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGACAGACACGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGGGCGG > minE/2356564‑2356678 | gagaCAGACACGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATgacgac > 1:1489653/1‑67 (MQ=255)gagaCAGACACGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGacgacg > 1:1060862/1‑68 (MQ=255)gagaCAGACACGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGacgacg > 1:2995597/1‑68 (MQ=255) cagacaCGTTAGCAGGGTCAATCCCACAATATAAGAGGCGATATCGGTGAATCTGATGACGACGATaaa < 1:1486393/69‑2 (MQ=255) cagacaCGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATaaa < 1:2221325/69‑2 (MQ=255) cagacaCGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATaaa < 1:380789/69‑2 (MQ=255) cagacaCGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATaaa < 1:3375455/69‑2 (MQ=255) tAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTtgt < 1:2063517/69‑1 (MQ=255) tAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTtgt < 1:3214397/69‑1 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATa > 1:1224033/1‑53 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACATGCGTtgtg > 1:1695304/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:1494543/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:2250958/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:2712657/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:151077/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:3280216/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:3425059/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:328378/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:1329738/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:1101013/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:621797/1‑65 (MQ=255) gggTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTtgtg > 1:53312/1‑65 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:948062/53‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:1137360/53‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:1828131/53‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:345811/53‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGt < 1:309932/59‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGt < 1:1225802/59‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGt < 1:2987595/59‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGt < 1:123824/59‑1 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCa > 1:249561/1‑69 (MQ=255) gTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCa > 1:848357/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:1889655/52‑1 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAAc < 1:2882150/52‑1 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGt < 1:2412435/58‑1 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:3021685/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:3242880/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:991854/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:2280581/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:2276634/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:2105295/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:1513072/1‑69 (MQ=255) tCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAg > 1:1485855/1‑69 (MQ=255) aaTCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATaaa < 1:3129400/50‑2 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:1905353/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:2814775/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:2974061/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:578118/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:1144121/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt > 1:2390313/1‑38 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGAt < 1:621842/38‑1 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:1447772/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:1569996/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:3091247/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:1771690/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:931386/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:1051250/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:102370/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:2881339/1‑66 (MQ=255) aTAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTaaa > 1:2279656/1‑66 (MQ=255) agagGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:2171052/69‑1 (MQ=255) agagGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:563708/69‑1 (MQ=255) agagGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:1800481/69‑1 (MQ=255) agagGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:2167132/69‑1 (MQ=255) agagGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:2551020/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:2541498/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:819440/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:844523/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:2592005/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:980045/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGGCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:1838447/69‑1 (MQ=255) gagGCGATATCGGTGAATCTGATGACGATGATAACAGCCGTTGTGCTGGCGGGCGGTAAAGCCAGACGa < 1:370230/69‑1 (MQ=255) agGCGATATCGGTGATTCTGATGACGACGATAACAGGGGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:3223502/69‑1 (MQ=255) agGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:190977/69‑1 (MQ=255) agGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:1208326/69‑1 (MQ=255) agGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:1392450/69‑1 (MQ=255) agGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:3422925/69‑1 (MQ=255) agGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGaa < 1:2695929/69‑1 (MQ=255) gATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:140503/63‑1 (MQ=255) gATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:2538487/63‑1 (MQ=255) tatCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:2431060/61‑1 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:2976374/1‑69 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:1643310/1‑69 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:2342489/1‑69 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:1204960/1‑69 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:1435461/1‑69 (MQ=255) atCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:3384491/1‑69 (MQ=255) atCGCTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:2088541/1‑69 (MQ=255) tCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:241732/1‑68 (MQ=255) tCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:2049384/1‑68 (MQ=255) tCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGggcgg > 1:3345156/1‑68 (MQ=255) gAAACTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:325913/54‑1 (MQ=255) acgacgATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACg < 1:1053985/44‑1 (MQ=255) | GAGACAGACACGTTAGCAGGGTCAATCCCACAATAAAAGAGGCGATATCGGTGAATCTGATGACGACGATAACAGGCGTTGTGCTGGCAGGCGGTAAAGCCAGACGAATGGGCGG > minE/2356564‑2356678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |