BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,359,866	T→A	40.3%	noncoding (1229/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,359,866	0	T	A	40.3%	142.0 / 140.8	151	noncoding (1229/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base T (69/21); new base A (51/10); total (120/31)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

TATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGC  >  minE/2359822‑2359932

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:3084566/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:2395271/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:2396235/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:2074512/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:2791972/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:2945858/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:1577010/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:1451251/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:3360407/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:1131114/1‑68 (MQ=11)

tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                             >  1:1146782/1‑68 (MQ=11)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:569405/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:1217575/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:1371674/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:2603799/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:3188969/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:149483/1‑68 (MQ=35)

  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:644065/68‑1 (MQ=255)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:2610926/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:2862248/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:1686215/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:2982497/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:2436708/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:2288187/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:1333921/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:1317491/68‑1 (MQ=11)

    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                         <  1:340087/68‑1 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:2077053/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:2599404/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:256009/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:2202514/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:1133951/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:271283/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:1546372/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:1164311/1‑68 (MQ=11)

     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                        >  1:3302997/1‑68 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:3029088/60‑1 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:1122156/60‑1 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:2168449/60‑1 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:1397196/60‑1 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:3424697/60‑1 (MQ=11)

          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:1231974/60‑1 (MQ=11)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:2633202/1‑69 (MQ=35)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:938518/1‑69 (MQ=35)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:3164784/1‑69 (MQ=35)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:136300/1‑69 (MQ=35)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:1264336/1‑69 (MQ=35)

           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                 >  1:1979509/1‑69 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              <  1:1012203/69‑1 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              >  1:154547/1‑69 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              >  1:878108/1‑69 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              <  1:181046/69‑1 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              >  1:2698356/1‑69 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              <  1:387043/69‑1 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              <  1:1033085/69‑1 (MQ=35)

              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                              >  1:891072/1‑69 (MQ=35)

                aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                            >  1:3245896/1‑69 (MQ=11)

                aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACATTCACAGGCTTACAGAACGCTcacc                            >  1:1307533/1‑69 (MQ=11)

                      cGCACTTCTGATACCTCCAGCATGCCTCACTGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:1617826/1‑68 (MQ=255)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                      >  1:2706018/1‑53 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:465190/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:485687/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:648698/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:3396878/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:2795971/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:3359/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:714963/1‑68 (MQ=35)

                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       >  1:3266157/1‑68 (MQ=35)

                            tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                <  1:3118446/69‑2 (MQ=11)

                             cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                >  1:3316545/1‑67 (MQ=11)

                             cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                >  1:2037778/1‑67 (MQ=11)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa            >  1:2510337/1‑65 (MQ=255)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt        >  1:3108664/1‑69 (MQ=255)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt        >  1:2301485/1‑69 (MQ=255)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt        >  1:3236693/1‑69 (MQ=255)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt        >  1:1757099/1‑69 (MQ=255)

                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt        >  1:2860896/1‑69 (MQ=255)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACg                                  >  1:326615/1‑41 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:775888/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:722703/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:2511091/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:2151649/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:782238/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:1893489/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:1867184/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:120668/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:2643662/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:1795919/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:3322226/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:330571/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:1627691/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:3031417/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:3057808/1‑42 (MQ=14)

                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                 >  1:149358/1‑42 (MQ=14)

                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa            >  1:573724/1‑59 (MQ=255)

                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:1452253/1‑69 (MQ=255)

                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:104122/1‑69 (MQ=255)

                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:1658619/1‑69 (MQ=255)

                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:2477373/1‑69 (MQ=255)

                                          cATGCCTCACAGCACACCTTCACAGGCGTACAGCACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:2218878/1‑69 (MQ=255)

TATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGC  >  minE/2359822‑2359932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`