Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,359,867 G→C 44.0% noncoding (1228/2905 nt) rrlA ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,359,8670GC44.0% 94.1 / 154.6 151noncoding (1228/2905 nt)rrlA23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (61/6);  new base C (51/10);  total (120/31)
Fisher's exact test for biased strand distribution p-value = 2.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 152 total aligned reads displayed.

TATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359822‑2359935
                                             |                                                                    
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:1451251/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:1131114/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:2396235/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:1146782/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:1577010/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:2701604/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:3360407/1‑68 (MQ=11)
tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAgg                                                >  1:3084566/1‑68 (MQ=11)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc                                               >  1:1626591/1‑67 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc                                               >  1:1847314/1‑67 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:149483/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:3104512/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:3188969/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:1371674/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:1682582/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:2042599/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:1217575/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:2604245/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:2427552/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:569405/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:2603799/1‑68 (MQ=35)
  tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:644065/68‑1 (MQ=255)
  tCGTTACTTATGTCAGCATTCGCACTTCTGAGACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                              >  1:3145594/1‑68 (MQ=25)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:1333921/68‑1 (MQ=11)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:1317491/68‑1 (MQ=11)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:1686215/68‑1 (MQ=11)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:340087/68‑1 (MQ=11)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:2982497/68‑1 (MQ=11)
    gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTa                                            <  1:2862248/68‑1 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:1546372/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:256009/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:1133951/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:1164311/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:271283/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:2077053/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:2931445/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:3019768/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:1759853/1‑68 (MQ=11)
     ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTCACAGGCTTAc                                           >  1:3302997/1‑68 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:1231974/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:2168449/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:3046725/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:3424697/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:2636012/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:1122156/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:2509189/60‑1 (MQ=11)
          tATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                              <  1:1397196/60‑1 (MQ=11)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca                                                  >  1:2763847/1‑55 (MQ=35)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                    >  1:2633202/1‑69 (MQ=35)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                    >  1:938518/1‑69 (MQ=35)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                    >  1:1264336/1‑69 (MQ=35)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                    >  1:136300/1‑69 (MQ=35)
           aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc                                    >  1:2127514/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 >  1:2564163/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 <  1:1012203/69‑1 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 >  1:891072/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 >  1:878108/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 <  1:1033085/69‑1 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 <  1:387043/69‑1 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 >  1:154547/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                 >  1:2698356/1‑69 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:1895878/1‑68 (MQ=35)
              tCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc                                  >  1:2219368/1‑68 (MQ=35)
                aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                               >  1:3245896/1‑69 (MQ=11)
                aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACATTCACAGGCTTACAGAACGCTcacc                               >  1:1307533/1‑69 (MQ=11)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:485687/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:3396878/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:3359/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:2795971/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:465190/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:3266157/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:3218946/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:648698/1‑68 (MQ=35)
                      cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                          >  1:714963/1‑68 (MQ=35)
                            tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                   <  1:3118446/69‑2 (MQ=11)
                             cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                   >  1:3316545/1‑67 (MQ=11)
                             cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                   >  1:2357840/1‑67 (MQ=11)
                             cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                   >  1:2037778/1‑67 (MQ=11)
                                    cTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt           >  1:2301485/1‑69 (MQ=255)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACg                                     >  1:326615/1‑41 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:3322226/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:722703/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:775888/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:782238/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:2511091/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:120668/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:330571/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:149358/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:2190161/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:1627691/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:1795919/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:3031417/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:1893489/1‑42 (MQ=14)
                                      ccAGCAACCCTCACAGGCCACCTTCACAGGCTTACAGAACGc                                    >  1:2807150/1‑42 (MQ=14)
                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa               >  1:573724/1‑59 (MQ=255)
                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc     >  1:1452253/1‑69 (MQ=255)
                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc     >  1:104122/1‑69 (MQ=255)
                                          cATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc     >  1:2477373/1‑69 (MQ=255)
                                          cATGCCTCACAGCACACCTTCACAGGCGTACAGCACGCTCCCCTACCCAACAACACATAGTGTCGCTGc     >  1:2218878/1‑69 (MQ=255)
                                             gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc  <  1:1854423/69‑1 (MQ=255)
                                             |                                                                    
TATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGC  >  minE/2359822‑2359935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑