| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,368,784 | C→T | 77.3% | I381I (ATC→ATT) | fadB → | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,368,784 | 0 | C | T | 77.3% | 42.2 / 7.4 | 35 | I381I (ATC→ATT) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (6/2); new base T (10/17); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGAACAAGCAGCTTGAGCGCGGCAAGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTT > minE/2368718‑2368841 | gctgAACAAGCAGCTTGAGCGCGGCAAGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCa < 1:1104910/69‑1 (MQ=255) aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCAGAATTCACCCAACGCTCGACTACGCCGGGttt < 1:2107447/69‑1 (MQ=255) aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1310956/69‑1 (MQ=255) aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1459754/69‑1 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1040851/68‑1 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGAttt > 1:503975/1‑68 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:1163726/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2952528/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2609505/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2547356/1‑69 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:468237/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:3000325/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2840122/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:234460/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2168927/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:511113/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:579788/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:1874574/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:1466281/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:934923/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:977662/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCGCGACTACGCCGGGTTTGa < 1:994982/69‑1 (MQ=255) aTCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2859864/68‑1 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCa > 1:2512623/1‑40 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:523760/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:825187/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:2453271/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:2108030/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1972981/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1760199/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1355434/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1327877/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1083495/1‑68 (MQ=255) tGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGata < 1:1880568/69‑1 (MQ=255) aTCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGgtt > 1:2482662/1‑69 (MQ=255) | GCTGAACAAGCAGCTTGAGCGCGGCAAGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTT > minE/2368718‑2368841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |