| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,368,808 | A→G | 63.9% | G389G (GGA→GGG) | fadB → | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,368,808 | 0 | A | G | 63.9% | 36.3 / 32.3 | 58 | G389G (GGA→GGG) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (12/9); new base G (9/28); total (21/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
AAGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGAAAAAAGC > minE/2368743‑2368867 | aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCAGAATTCACCCAACGCTCGACTACGCCGGGttt < 1:2107447/69‑1 (MQ=255)aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1310956/69‑1 (MQ=255)aaGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1459754/69‑1 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGttt < 1:1040851/68‑1 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGAttt > 1:503975/1‑68 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2547356/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2609505/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:1163726/1‑69 (MQ=255) aGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTg > 1:2952528/1‑69 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2168927/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:511113/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:579788/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:234460/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:1874574/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:468237/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:1466281/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:3000325/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2840122/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:934923/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:977662/69‑1 (MQ=255) gATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCGCGACTACGCCGGGTTTGa < 1:994982/69‑1 (MQ=255) aTCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGa < 1:2859864/68‑1 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:523760/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:2108030/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1972981/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1760199/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1355434/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1327877/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:2453271/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:825187/1‑68 (MQ=255) tGGTCTGAAACTGGCTGGCGTGATCTCCACAATTCACCCAACGCTCGACTACGCCGGGTTTGACcgcg > 1:1083495/1‑68 (MQ=255) tGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGata < 1:1880568/69‑1 (MQ=255) aTCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGgtt > 1:2482662/1‑69 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:2608145/69‑1 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:365776/69‑1 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:3378632/69‑1 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:1110861/69‑1 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:305797/69‑1 (MQ=255) cccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:2994369/69‑1 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATtgt > 1:1097065/1‑41 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaaa > 1:2444126/1‑69 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaaa > 1:3269832/1‑69 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaaa > 1:2838055/1‑69 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaaa > 1:2420718/1‑69 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaa < 1:1093109/68‑1 (MQ=255) ccAACGCTCGACTACGCCGGATTTGACAGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGaaa > 1:100157/1‑69 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTTTTGAAAACCCGAAAGTGaaaa < 1:2238829/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAATTGaaaa < 1:3649/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:1027941/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:785278/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:2004492/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:2108912/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:2239766/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:3191383/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:245142/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:2514123/69‑1 (MQ=255) tCGACTACGCCGGGTTTGACCGCGTGGATGTTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGaaaa < 1:2536432/69‑1 (MQ=255) cTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGAAAAAAGc < 1:39911/69‑1 (MQ=255) | AAGATCGATGGTCTGAAACTGGCTGGCGTGATCTCCACAATCCACCCAACGCTCGACTACGCCGGATTTGACCGCGTGGATATTGTGGTAGAAGCGGTTGTTGAAAACCCGAAAGTGAAAAAAGC > minE/2368743‑2368867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |