| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,369,021 | G→A | 76.8% | L460L (TTG→TTA) | fadB → | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,369,021 | 0 | G | A | 76.8% | 72.4 / 17.7 | 60 | L460L (TTG→TTA) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (10/4); new base A (20/26); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCTGGAACGCCCGGAAAACTTCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGAGCAA > minE/2368958‑2369089 | gcTGGAACGCCCGGAAAACTTCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGa < 1:2004131/69‑1 (MQ=255) cTGGAACGCCCGGAAAACTTCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGa < 1:2078232/68‑1 (MQ=255) aaCTTCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCgag < 1:2659177/69‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:876410/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1019684/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:2521034/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:913723/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:2359527/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:2931269/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:2976820/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1830213/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:446945/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1509488/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1362401/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:52559/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:825130/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:135934/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1268161/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1113003/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:630827/54‑1 (MQ=255) tCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:1115754/54‑1 (MQ=255) cTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGAAatta < 1:2812948/53‑1 (MQ=255) cACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGaa > 1:932399/1‑69 (MQ=255) cACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGaa > 1:195740/1‑69 (MQ=255) cACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGaa > 1:1222829/1‑69 (MQ=255) cACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGaa > 1:738849/1‑69 (MQ=255) aCTTCTTTAACCCGGTCCACCGAATGCCGTTAGTAGATatta < 1:1715002/42‑1 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:254709/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:2834154/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:284983/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:3087430/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:596048/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:3125453/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:3311788/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:3392686/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:926369/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:1069276/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:1090254/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:959251/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:1501210/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:1665160/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:919733/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:2323715/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:2622132/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:2382058/1‑51 (MQ=255) cttTAACCCGGTCCACCGAATGCCGTTAGTAGAAATAATTCGCGGCGAGaa > 1:1305552/1‑51 (MQ=255) cttTAACCCGGTCAACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGaa > 1:1587532/1‑51 (MQ=255) ccGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGaaa < 1:3345192/69‑1 (MQ=255) tCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:2951936/69‑3 (MQ=255) tCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:2781882/69‑3 (MQ=255) tCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:906488/69‑3 (MQ=255) tCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:190262/69‑3 (MQ=255) tCCACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:306670/69‑3 (MQ=255) ccACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATcgc > 1:1114317/1‑60 (MQ=255) ccACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGt > 1:3365763/1‑69 (MQ=255) ccACCGAATGCCATTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGt > 1:3087126/1‑69 (MQ=255) cACCGAATGCCGTTAGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGtcg < 1:2777907/67‑3 (MQ=255) tGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTgg > 1:2469461/1‑68 (MQ=255) tGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTgg > 1:2234608/1‑68 (MQ=255) tGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTgg > 1:2952478/1‑68 (MQ=255) ggTTGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:1805167/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:2688705/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:2852820/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:2740188/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:2130501/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGa > 1:1364220/1‑65 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGAGCaa < 1:3291249/69‑1 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGAGCaa < 1:1153365/69‑1 (MQ=255) ggTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGAGCaa < 1:987194/69‑1 (MQ=255) | GCTGGAACGCCCGGAAAACTTCTGCGGGATGCACTTCTTTAACCCGGTCCACCGAATGCCGTTGGTAGAAATTATTCGCGGCGAGAAAAGCTCCGACGAAACCATCGCGAAAGTTGTCGCCTGGGCGAGCAA > minE/2368958‑2369089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |