| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,369,345 | C→T | 41.0% | Y568Y (TAC→TAT) | fadB → | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,369,345 | 0 | C | T | 41.0% | 15.9 / 30.0 | 47 | Y568Y (TAC→TAT) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (11/16); new base T (15/4); total (27/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01 | |||||||||||
ATTGATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTT > minE/2369277‑2369407 | aTTGATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTAc > 1:3107345/1‑69 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc > 1:3169200/1‑69 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc < 1:2947032/69‑1 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc < 1:512203/69‑1 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc < 1:2611284/69‑1 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc < 1:2597881/69‑1 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc > 1:540492/1‑69 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc > 1:628611/1‑69 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc < 1:1308427/69‑1 (MQ=255) gATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACcgc > 1:1380228/1‑69 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:1017520/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:1720250/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:95026/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:3115188/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:1346062/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGcc < 1:2418064/69‑1 (MQ=255) gcgcATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGc < 1:3059809/68‑1 (MQ=255) cAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTg > 1:316325/1‑69 (MQ=255) gCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGttt < 1:569179/69‑1 (MQ=255) gCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGttt < 1:3128914/69‑1 (MQ=255) gCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGttt < 1:1493067/69‑1 (MQ=255) cATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGttt < 1:1263899/64‑1 (MQ=255) ggCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCaa > 1:2549185/1‑69 (MQ=255) gcagcaGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAAc > 1:1570903/1‑69 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:545761/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:2019229/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:3158792/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:2966025/69‑1 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:424478/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:2937383/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:2665202/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:1218301/1‑69 (MQ=255) aGATTAACGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:442899/1‑69 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:905400/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:957317/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:982027/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2847426/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:416959/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:3334407/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:26785/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2478603/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2213119/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2025818/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2024998/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2004314/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:1950839/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:1100751/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGACt > 1:1025709/1‑60 (MQ=255) | ATTGATACCGCGCATCACGCTCAGGCTGTCATGGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTT > minE/2369277‑2369407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |