| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,369,370 | G→A | 52.8% | D577N (GAT→AAT) | fadB → | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,369,370 | 0 | G | A | 52.8% | ‑2.6 / 28.7 | 36 | D577N (GAT→AAT) | fadB | fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase and 3‑hydroxyacyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (7/10); new base A (15/4); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCAAA > minE/2369309‑2369432 | ggCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCaa > 1:2549185/1‑69 (MQ=255) gcagcaGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAAc > 1:1570903/1‑69 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:545761/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:2019229/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:3158792/69‑1 (MQ=255) cGGATGCAGAAAGATTATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAAc < 1:2966025/69‑1 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:2665202/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:1218301/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:424478/1‑69 (MQ=255) aGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:2937383/1‑69 (MQ=255) aGATTAACGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGttt > 1:442899/1‑69 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:3334407/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:26785/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2847426/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:982027/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:416959/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:905400/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:957317/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2478603/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2213119/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:1100751/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2025818/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2024998/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:2004314/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGCCt > 1:1950839/1‑60 (MQ=255) ttATCGCGATGCCATCGACGCGCTGTTTAATGCCAACCGCTTTGGTCAGAAGAACGGACt > 1:1025709/1‑60 (MQ=255) ccATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAaag < 1:2492583/69‑1 (MQ=255) ccATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAaag < 1:2736363/69‑1 (MQ=255) ccATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAaag < 1:1832883/69‑1 (MQ=255) ccATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAaag < 1:1283554/69‑1 (MQ=255) ccATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAaag < 1:1157161/69‑1 (MQ=255) cTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCaaa < 1:1020320/69‑1 (MQ=255) cTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCaaa < 1:2493037/69‑1 (MQ=255) cTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCaaa < 1:2030980/69‑1 (MQ=255) cTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCaaa < 1:2729988/69‑1 (MQ=255) cTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAACAAGACAGCaaa < 1:2579586/69‑1 (MQ=255) | GGCAGCAGGCTTCCCGCAGCGGATGCAGAAAGATTACCGCGATGCCATCGACGCGCTGTTTGATGCCAACCGCTTTGGTCAGAAGAACGGCCTCGGTTTCTGGCGTTATAAAGAAGACAGCAAA > minE/2369309‑2369432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |