| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,377,653 | G→A | 20.7% | R245R (CGC→CGT) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,377,653 | 0 | G | A | 20.7% | 134.4 / 23.1 | 91 | R245R (CGC→CGT) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base G (41/31); new base A (8/11); total (49/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01 | |||||||||||
CAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGATGTAGAGC > minE/2377587‑2377713 | cAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:1145573/69‑1 (MQ=255)cAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:281103/69‑1 (MQ=255)cAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:19449/69‑1 (MQ=255)cAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:1458299/69‑1 (MQ=255)cAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:1442682/69‑1 (MQ=255) tttgTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:286635/4‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:58394/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:1601649/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:326249/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:2557997/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:1504535/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:2469356/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:1902921/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:547035/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:1236162/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:1218131/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:534357/1‑69 (MQ=255) ttAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTccatcaccatc > 1:456924/1‑69 (MQ=255) gctgTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATccc < 1:1466400/66‑1 (MQ=255) ccGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATccc > 1:1023231/1‑68 (MQ=255) ccGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATccc > 1:254767/1‑68 (MQ=255) ccGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATccc > 1:306253/1‑68 (MQ=255) ccGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATccc > 1:3142068/1‑68 (MQ=255) ttttCTCCAGCGTTGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:2058511/1‑69 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCa > 1:681017/1‑68 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCa > 1:2899432/1‑68 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:821217/1‑69 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:3327206/1‑69 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:1849843/1‑69 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:1242161/1‑69 (MQ=255) ttttCTCCAGCGTCGCAACATCAGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCAc > 1:2267879/1‑69 (MQ=255) tctcCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATgcg < 1:1563111/43‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2481329/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1894779/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:3370967/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2993215/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2875238/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2829641/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2598033/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2775032/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:3252956/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2025060/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:115354/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1280910/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:823424/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1505744/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:784290/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1663063/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1667021/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1704787/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1881861/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:659698/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1873934/69‑1 (MQ=255) aaCATCAGACACCGGAATGCCGTAAATGCGCTCCATAACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:618577/69‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:3104470/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1263341/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:86090/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:1603097/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:3319444/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa < 1:2684561/65‑1 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAAc > 1:2970424/1‑68 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACt > 1:1728677/1‑69 (MQ=255) tCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACt > 1:2628627/1‑69 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:89011/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:921376/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:3129882/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:38834/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:1595965/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:2797829/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:2757761/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:2547058/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:2224963/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:1332600/69‑1 (MQ=255) aGACACCGGAATGCCGTAAATACGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTc < 1:2091225/69‑1 (MQ=255) cacCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCgg > 1:1517767/1‑69 (MQ=255) cacCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCgg > 1:344376/1‑69 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTc > 1:367856/1‑50 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGa > 1:2923424/1‑67 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGa > 1:1776078/1‑67 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGa > 1:2066765/1‑67 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGa > 1:488150/1‑67 (MQ=255) ggAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGa > 1:280756/1‑67 (MQ=255) ccGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGATGTAGAGc < 1:2153293/69‑1 (MQ=255) ccGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGATGTAGAGc < 1:1960327/69‑1 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGt > 1:134298/1‑44 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:869270/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:849886/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:2969024/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:1366813/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:1096081/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:1462694/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:1771984/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:1851436/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:492691/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:415497/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:3392464/1‑45 (MQ=255) aaTGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTa > 1:328727/1‑45 (MQ=255) | CAGCAATTTCATGTTAGTGCCGTTTTTCTCCAGCGCCGCAACATCAGACACCGGAATGCCGTAAATGCGCTCCATCACCATCATCCCTTCACTACAATAGTCAGGGTAAACTTCCGGGATGTAGAGC > minE/2377587‑2377713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |