| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,378,139 | T→C | 41.9% | P83P (CCA→CCG) | ubiB ← | 2‑octaprenylphenol hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,378,139 | 0 | T | C | 41.9% | 16.7 / 45.0 | 62 | P83P (CCA→CCG) | ubiB | 2‑octaprenylphenol hydroxylase |
| Reads supporting (aligned to +/- strand): ref base T (17/19); new base C (11/15); total (28/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
TCGCCAGCTTGCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGCCCCAGTTCTTGCA > minE/2378072‑2378206 | tCGCCAGCTTGCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGt > 1:3053372/1‑68 (MQ=255)tCGCCAGCTTGCCATCAAACGGAGAAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGt > 1:2874296/1‑68 (MQ=255) tGCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGAt > 1:539827/1‑69 (MQ=255) gCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATc > 1:1151664/1‑69 (MQ=255) gCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATc > 1:2127867/1‑69 (MQ=255) gCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATc > 1:2131875/1‑69 (MQ=255) aCGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCggg > 1:870107/1‑69 (MQ=255) aCGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCggg > 1:1608927/1‑69 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGTCGGaaa < 1:5417/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGTaaa < 1:2808308/53‑1 (MQ=38) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:595053/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:2018492/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:2022194/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:2700014/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:867093/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:575731/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:3177911/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:443911/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:468335/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:1995505/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:2014602/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGaaa < 1:970259/53‑1 (MQ=255) ggAGCGACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGAGGCGGaaa < 1:2197104/53‑1 (MQ=255) ttGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACAtt > 1:809793/1‑69 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:1024256/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:473368/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:1282995/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:3050379/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:990107/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:1498291/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:2817896/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:2681585/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:2449564/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGcc > 1:234372/1‑68 (MQ=255) cTGCAATAACGCCAGCTGATCGGCAATATGCGGCGGAAAAAGATCGCGGCGGGTTGATAACATCTGc > 1:1681144/1‑67 (MQ=255) aaCGCCATCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:1232828/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAAc < 1:2032273/54‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAAc < 1:1115832/54‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAAc < 1:515434/54‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAAc < 1:1310305/54‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:1335455/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:1915610/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:3170121/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:2638241/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:1521164/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:1711190/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:261468/69‑1 (MQ=255) aaCGCCAGCTGATCGGCAATATCCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTg < 1:2607642/69‑1 (MQ=255) aCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGa > 1:1054541/1‑69 (MQ=255) aCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGa > 1:857941/1‑69 (MQ=255) aCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGa > 1:3392402/1‑69 (MQ=255) aCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGa > 1:3017250/1‑69 (MQ=255) aCGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGa > 1:19626/1‑69 (MQ=255) aGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:3175909/69‑1 (MQ=255) aGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:3093443/69‑1 (MQ=255) aGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:3075101/69‑1 (MQ=255) aGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:2401279/69‑1 (MQ=255) aGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:1900526/69‑1 (MQ=255) gCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCaa < 1:2574906/68‑1 (MQ=255) cGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGc > 1:2334663/1‑68 (MQ=255) cGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGc > 1:997139/1‑68 (MQ=255) atGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGCCCCAGTTc > 1:3001701/1‑69 (MQ=255) atGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGCCCCAGTTc > 1:1380625/1‑69 (MQ=255) gTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGCCCCAGTTCTTGCa > 1:495779/1‑69 (MQ=255) | TCGCCAGCTTGCCATCAAACGGAGCAACTTTGTCCTGCAATAACGCCAGCTGATCGGCAATATGCGGTGGAAAAAGATCGCGGCGGGTTGATAACATTTGCCCGAACTTGATCCAAACCGGCCCCAGTTCTTGCA > minE/2378072‑2378206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |