| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,384,433 | G→A | 62.4% | A376A (GCC→GCT) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,384,433 | 0 | G | A | 62.4% | 58.2 / 71.2 | 109 | A376A (GCC→GCT) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/34); new base A (45/23); total (52/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.32e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
CAGACGCTTTTCTACCGCCGGATTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTT > minE/2384373‑2384497 | cAGACGCTTTTCTACCGCCGGATTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCa < 1:746934/69‑1 (MQ=255) aGACGCTTTTCTACCGCCGGATTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCa < 1:509640/68‑1 (MQ=255) cTTTTCTACCGCCGGATTATGTACTCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGc < 1:2449721/69‑1 (MQ=255) ttATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTc > 1:2688981/1‑68 (MQ=255) tATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTTCCAGAGCTGCCGTGTcac < 1:889212/69‑1 (MQ=255) tATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTcac < 1:2114753/69‑1 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:1100307/1‑68 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:537693/1‑68 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:684275/1‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGCATCGGAGCGCTCCActc > 1:3309604/3‑40 (MQ=21) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTg > 1:677050/3‑42 (MQ=37) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:1225691/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:2827367/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:192924/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:2458030/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:1675073/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2258163/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2075871/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2313119/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2350687/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2525020/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2485390/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:623790/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2569832/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2676352/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:818742/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:3171284/3‑69 (MQ=255) ggTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:353827/69‑1 (MQ=255) gTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:2748713/68‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:58112/69‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:2543751/69‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:3189019/69‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:316537/69‑1 (MQ=255) cgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:3134861/68‑1 (MQ=255) cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:2750741/69‑1 (MQ=255) cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:3173894/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGGCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1043159/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCTGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1806855/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1564577/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1474263/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:3180275/69‑1 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2025025/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2570648/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:460003/1‑68 (MQ=255) tGAATCGTAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2456861/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACTCATCGCGCAGCAATGcc < 1:2691258/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2832076/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1323286/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2670592/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:3173104/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1544387/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2225330/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:675024/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1873636/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1931304/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1992821/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2002631/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:537219/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:371147/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:375238/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2080915/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2254476/69‑1 (MQ=255) tGAATCGGAGCGCTACACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:898860/69‑1 (MQ=255) gAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1388945/68‑1 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:915625/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1030723/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:428748/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:75447/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:58626/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2275473/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1547434/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1641733/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1650004/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1677807/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1545984/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2010083/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1398881/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2269511/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2272616/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2332204/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1046215/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:3045893/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2561458/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2633215/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2851264/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2885997/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGATCAACGCATcg > 1:2801065/1‑54 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGTAGTGCCAGtt < 1:743517/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1060171/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1042581/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1054153/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1325541/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1805521/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2129274/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2236404/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:365368/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3389613/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3268265/69‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1946987/68‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2515399/68‑1 (MQ=255) | CAGACGCTTTTCTACCGCCGGATTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTT > minE/2384373‑2384497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |