| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,384,460 | G→A | 61.1% | D367D (GAC→GAT) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,384,460 | 0 | G | A | 61.1% | 51.0 / 73.9 | 108 | D367D (GAC→GAT) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/35); new base A (43/23); total (50/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCA > minE/2384395‑2384523 | ttATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTc > 1:2688981/1‑68 (MQ=255) tATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTTCCAGAGCTGCCGTGTcac < 1:889212/69‑1 (MQ=255) tATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTcac < 1:2114753/69‑1 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:537693/1‑68 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:1100307/1‑68 (MQ=255) cgcgGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTg > 1:684275/1‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:1675073/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:237381/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:2458030/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:1225691/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTc > 1:2827367/3‑68 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:3171284/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2569832/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2075871/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2258163/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2668622/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2485390/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:345140/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:623790/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2676352/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:1450387/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2313119/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:818742/3‑69 (MQ=255) gagTAGAGTGACGACGCGCCTGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCa > 1:2350687/3‑69 (MQ=255) ggTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:353827/69‑1 (MQ=255) gTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg < 1:2748713/68‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:58112/69‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:316537/69‑1 (MQ=255) acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:2543751/69‑1 (MQ=255) cgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg < 1:3134861/68‑1 (MQ=255) cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:3173894/69‑1 (MQ=255) cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc < 1:2750741/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGGCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1043159/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCTGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1806855/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:3180275/69‑1 (MQ=255) gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1564577/69‑1 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2025025/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2570648/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:460003/1‑68 (MQ=255) tGAATCGTAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2456861/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACTCATCGCGCAGCAATGcc < 1:2691258/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2977538/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2670592/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2832076/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1931304/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1323286/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1544387/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:675024/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:537219/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1873636/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:3173104/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1992821/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2002631/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:375238/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2080915/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2121573/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:371147/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2225330/69‑1 (MQ=255) tGAATCGGAGCGCTACACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:898860/69‑1 (MQ=255) gAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1388945/68‑1 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:915625/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1030723/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:75447/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:58626/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:3045893/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:428748/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2851264/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1650004/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1677807/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1641733/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1547434/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1545984/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2010083/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1398881/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2269511/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2272616/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2275473/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2332204/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:1046215/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2561458/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2633215/1‑54 (MQ=255) aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg > 1:2885997/1‑54 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGTAGTGCCAGtt < 1:743517/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1054153/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1060171/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1325541/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1042581/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1805521/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1812269/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2129274/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:365368/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2236404/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3389613/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3268265/69‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1946987/68‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2515399/68‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:65973/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:723924/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:914856/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:265513/69‑1 (MQ=255) | TTATGTACGCGGGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCA > minE/2384395‑2384523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |