Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,384,474 G→A 64.1% L363L (CTG→TTG)  metE ← 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,384,4740GA64.1% 62.6 / 63.0 103L363L (CTG→TTG) metE5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (5/32);  new base A (43/23);  total (48/55)
Fisher's exact test for biased strand distribution p-value = 3.84e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

Only 100 of 105 total aligned reads displayed.

GGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTC  >  minE/2384406‑2384535
                                                                    |                                                             
ggTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg                                                               <  1:353827/69‑1 (MQ=255)
 gTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAg                                                               <  1:2748713/68‑1 (MQ=255)
         acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg                                                      <  1:3189019/69‑1 (MQ=255)
         acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg                                                      <  1:316537/69‑1 (MQ=255)
         acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg                                                      <  1:2543751/69‑1 (MQ=255)
         acgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg                                                      <  1:58112/69‑1 (MQ=255)
          cgacgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATcgcg                                                      <  1:3134861/68‑1 (MQ=255)
             cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc                                                  <  1:3173894/69‑1 (MQ=255)
             cgTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCgcagc                                                  <  1:2750741/69‑1 (MQ=255)
                gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGGCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               <  1:1043159/69‑1 (MQ=255)
                gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCTGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               <  1:1806855/69‑1 (MQ=255)
                gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               <  1:3180275/69‑1 (MQ=255)
                gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               <  1:1474263/69‑1 (MQ=255)
                gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               <  1:1564577/69‑1 (MQ=255)
                 ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               >  1:2570648/1‑68 (MQ=255)
                 ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt                                               >  1:2025025/1‑68 (MQ=255)
                   tGAATCGTAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2456861/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACTCATCGCGCAGCAATGcc                                            <  1:2691258/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2121573/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2080915/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2225330/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2670592/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2002631/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:675024/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1992821/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:2977538/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1931304/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1544387/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:537219/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:375238/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:371147/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1323286/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:3173104/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:603508/69‑1 (MQ=255)
                   tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1873636/69‑1 (MQ=255)
                   tGAATCGGAGCGCTACACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:898860/69‑1 (MQ=255)
                    gAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc                                            <  1:1388945/68‑1 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:3045893/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:58626/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2851264/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2272616/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2633215/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2885997/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2561458/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:428748/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1547434/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2332204/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2275473/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1046215/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2269511/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1641733/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1677807/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1030723/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1545984/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:1398881/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:75447/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:2010083/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATcg                                                        >  1:915625/1‑54 (MQ=255)
                      aTCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGATCAACGCATcg                                                        >  1:2801065/1‑54 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGTAGTGCCAGtt                                        <  1:743517/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:3268265/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1325541/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1805521/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:3389613/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:365368/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1060171/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1054153/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1042581/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1812269/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:2129274/69‑1 (MQ=255)
                       tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:2236404/69‑1 (MQ=255)
                        cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:1946987/68‑1 (MQ=255)
                        cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt                                        <  1:2515399/68‑1 (MQ=255)
                                                 gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa              <  1:914856/69‑1 (MQ=255)
                                                 gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa              <  1:65973/69‑1 (MQ=255)
                                                 gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa              <  1:723924/69‑1 (MQ=255)
                                                 gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa              <  1:265513/69‑1 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCCGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:541092/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:698449/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:621657/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:652246/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:95710/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:989854/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:4610/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1072375/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1098774/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1606479/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1778753/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1866971/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:1926499/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:2235025/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:2587076/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:2846979/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:2864477/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:3163558/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt      >  1:511112/1‑69 (MQ=255)
                                                         accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTATTGCAGGGCGAAGGCAAACCAGCt      >  1:1183079/1‑69 (MQ=255)
                                                            acTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt   >  1:2687263/1‑69 (MQ=255)
                                                            acTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt   >  1:2691100/1‑69 (MQ=255)
                                                               gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTc  <  1:2385407/67‑1 (MQ=255)
                                                                    |                                                             
GGTAGAGTGACGACGTGCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTC  >  minE/2384406‑2384535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: