| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,384,489 | G→A | 57.3% | L358L (CTG→TTG) | metE ← | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,384,489 | 0 | G | A | 57.3% | 18.5 / 57.3 | 75 | L358L (CTG→TTG) | metE | 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (7/25); new base A (20/23); total (27/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCA > minE/2384422‑2384544 | gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGGCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1043159/69‑1 (MQ=255)gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCTGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1806855/69‑1 (MQ=255)gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:3180275/69‑1 (MQ=255)gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1564577/69‑1 (MQ=255)gCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:1474263/69‑1 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt < 1:992183/68‑1 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:460003/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2570648/1‑68 (MQ=255) ccTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGt > 1:2025025/1‑68 (MQ=255) tGAATCGTAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2456861/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACTCATCGCGCAGCAATGcc < 1:2691258/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2002631/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2670592/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2832076/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2977538/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:3173104/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2254476/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2225330/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2121573/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:2080915/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:371147/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1992821/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1931304/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:375238/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1544387/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1873636/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:537219/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1323286/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:603508/69‑1 (MQ=255) tGAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:675024/69‑1 (MQ=255) tGAATCGGAGCGCTACACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:898860/69‑1 (MQ=255) gAATCGGAGCGCTCCACTCTGCCAGAGCTGCCGTATCACCACTGTTCAACGCATCGCGCAGCAATGcc < 1:1388945/68‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGTAGTGCCAGtt < 1:743517/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3268265/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1060171/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:3389613/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1042581/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:365368/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1054153/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1325541/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1805521/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1812269/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2129274/69‑1 (MQ=255) tCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2236404/69‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:1946987/68‑1 (MQ=255) cGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGtt < 1:2515399/68‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:265513/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:914856/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:723924/69‑1 (MQ=255) gCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCa < 1:65973/69‑1 (MQ=255) accacTGTTCAACGCATCGCGCCGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:541092/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1866971/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1926499/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:621657/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:652246/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:698449/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1778753/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:95710/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:989854/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1606479/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:511112/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:4610/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1098774/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:2235025/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:3163558/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:2864477/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:2846979/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:1072375/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTTTTGCAGGGCGAAGGCAAACCAGCt > 1:2587076/1‑69 (MQ=255) accacTGTTCAACGCATCGCGCAGCAATGCCAGTTCATGACATTATTGCAGGGCGAAGGCAAACCAGCt > 1:1183079/1‑69 (MQ=255) acTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt > 1:2691100/1‑69 (MQ=255) acTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCtttt > 1:2687263/1‑69 (MQ=255) gTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTc < 1:2385407/67‑1 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCa > 1:2338028/1‑66 (MQ=255) tCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCa > 1:2672639/1‑66 (MQ=255) | GCCTGAATCGGGGCGCTCCACTCTGCCAGAGCTGCCGTGTCACCACTGTTCAGCGCATCGCGCAGCAGTGCCAGTTCATGGCATTTTTGTAGGGCGAAGGCAAACCAGCTTTTCACTTCTGCA > minE/2384422‑2384544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |