| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,386,995 | A→G | 33.4% | F181F (TTT→TTC) | yigM ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,386,995 | 0 | A | G | 33.4% | 148.6 / 110.3 | 191 | F181F (TTT→TTC) | yigM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (75/52); new base G (36/28); total (111/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
Only 100 of 192 total aligned reads displayed.
TGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCATCAGGC > minE/2386930‑2387059 | tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:2269489/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:892607/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:2971556/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:1122025/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:562791/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:644411/1‑69 (MQ=255)tGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAc > 1:630546/1‑69 (MQ=255) gCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAcg > 1:2571431/1‑69 (MQ=255) gCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAAcg > 1:2487551/1‑69 (MQ=255) tGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCaa < 1:2210230/69‑1 (MQ=255) tGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCaa < 1:1769611/69‑1 (MQ=255) tGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCaa < 1:3226584/69‑1 (MQ=255) tGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCaa < 1:619505/69‑1 (MQ=255) tGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCaa < 1:758085/69‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:2069421/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:167255/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:2791724/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:223446/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:3155528/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:79219/68‑1 (MQ=255) cGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGa < 1:1926877/68‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:324706/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:2662163/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:2060486/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:1607301/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:1551728/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCa < 1:855717/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCAc > 1:773315/1‑66 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:2812741/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:187847/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:2703694/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:537022/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:2130151/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:1555704/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACg > 1:675186/1‑67 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCa < 1:2432102/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCa < 1:1024314/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCa < 1:2686479/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCa < 1:923776/69‑1 (MQ=255) ttCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCa < 1:2264198/69‑1 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1722431/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:2951874/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:162099/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:2555130/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:3354898/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:796910/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1326844/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1428865/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGc > 1:3093357/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:2134890/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1872897/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:2882413/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:403075/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1570781/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:1018741/1‑68 (MQ=255) gcATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGc > 1:2334636/1‑68 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:3426618/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:190632/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:327972/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:520462/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:29909/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:2822404/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:1488145/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAgg < 1:1083689/66‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:3400560/69‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:230564/69‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:2424099/69‑1 (MQ=255) cATTTCCCAGCAAGAACCATGCAATCACTGCCA‑CAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:96095/68‑1 (MQ=255) aTTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:676218/68‑1 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:3314576/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:1095892/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:1938131/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:2907418/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:3002193/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:902071/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:2454924/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:395907/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:3136034/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtgc > 1:3190049/1‑67 (MQ=255) gCAAGAACCATGCAATCACTGCCACCAGGAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCtg > 1:1984805/1‑67 (MQ=255) cAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAg > 1:373638/1‑69 (MQ=255) cAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAg > 1:3417409/1‑69 (MQ=255) cAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAg > 1:2610601/1‑69 (MQ=255) cAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAg > 1:1392425/1‑69 (MQ=255) cACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTc > 1:881383/1‑69 (MQ=255) cACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTc < 1:2224368/69‑1 (MQ=255) aCTGCCACCAGAAACGCGCCAGGATAGAACCACGCAAAGGCGt < 1:1760893/43‑1 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt > 1:1155559/1‑43 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt > 1:2343540/1‑43 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt > 1:766107/1‑43 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:1309653/43‑1 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt > 1:637387/1‑43 (MQ=255) aCTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGt < 1:2722564/43‑1 (MQ=255) tGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCa < 1:633448/68‑1 (MQ=255) tGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCa < 1:3041351/68‑1 (MQ=255) ccaGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCATCAGGc > 1:2606887/1‑69 (MQ=255) ccaGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCATCAGGc > 1:1051243/1‑69 (MQ=255) ccaGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCATCAGGc > 1:397663/1‑69 (MQ=255) gAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGc > 1:575831/1‑44 (MQ=255) gAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGc > 1:1825180/1‑44 (MQ=255) | TGCAGCGTGGTTTGCGGCATTTTCTGCGCATTTCCCAGCAAGAACCATGCAATCACTGCCACCAGAAACGCGCCAAGATAGAACCACGCAAAGGCGTTATGCTGTGGCATCGGGCGAGTTTCCATCAGGC > minE/2386930‑2387059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |