| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,387,114 | G→A | 34.6% | L142L (CTG→TTG) | yigM ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,387,114 | 0 | G | A | 34.6% | 55.3 / 39.9 | 75 | L142L (CTG→TTG) | yigM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (33/16); new base A (17/9); total (50/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
TCCATCAGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCACC > minE/2387050‑2387175 | tCCATCAGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCaa > 1:2546024/1‑68 (MQ=255) aGGCGTTTGTAACCCACCATGCCAATGGCGAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAg > 1:2813846/1‑67 (MQ=255) aGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAg > 1:3152145/1‑67 (MQ=255) aGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAg > 1:2834663/1‑67 (MQ=255) aGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAg > 1:2761875/1‑67 (MQ=255) aGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAg > 1:1739601/1‑67 (MQ=255) aGGCGTTTGTAACCCA‑CATGCCAATGGCAAAAGTGATATTGGAGAGTTGCCCCAGCAGCAAGCCAg > 1:2886674/1‑66 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:751668/69‑1 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:1176843/69‑1 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:3342979/69‑1 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:3092486/69‑1 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:3088422/69‑1 (MQ=255) ccaTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:2924671/69‑1 (MQ=255) aTGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGa < 1:2349188/67‑1 (MQ=255) ggcAATGGCAAAAGTGATGTTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg < 1:472285/67‑1 (MQ=255) gCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg < 1:1101945/69‑1 (MQ=255) ccAATGGCAAAAGTGATATTGGGGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg > 1:1907723/1‑68 (MQ=255) ccAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCt > 1:528101/1‑67 (MQ=255) ccAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg > 1:1504033/1‑68 (MQ=255) ccAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg > 1:2159386/1‑68 (MQ=255) ccAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTg > 1:1579256/1‑68 (MQ=255) gCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATa > 1:171865/1‑68 (MQ=255) gCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATa > 1:872971/1‑68 (MQ=255) gCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATa > 1:1526984/1‑68 (MQ=255) gATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGaataat < 1:911875/69‑1 (MQ=255) gATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGaataat < 1:1483837/69‑1 (MQ=255) gATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGaataat < 1:3006200/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:181761/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:451156/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:2660901/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:564299/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:688034/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:3131260/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:2520192/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:2487754/69‑1 (MQ=255) atatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc < 1:1885327/69‑1 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTc > 1:3183415/1‑43 (MQ=37) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:3334374/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:314050/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:1255578/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:1265782/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:3173761/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:1756317/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2110553/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2788295/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2664744/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:562031/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:607291/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2452007/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2295618/1‑69 (MQ=255) tatTGGAGAGTTGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATc > 1:1537079/1‑68 (MQ=255) tatTGGAGAGTAGCACCAGCAATAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATcc > 1:2171257/1‑69 (MQ=255) tatTGCAGAGTTGCACCAGCAATAAGCCAGTCCAg > 1:1233640/1‑35 (MQ=18) ttGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATccc > 1:621408/1‑68 (MQ=255) ttGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATccc > 1:184661/1‑68 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATg < 1:860017/36‑1 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATg < 1:3303805/36‑1 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACTTGATCATAGCGAATAATCCCGGc > 1:1730564/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCgg > 1:1548192/1‑68 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:3301619/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:2294438/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:761796/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:701698/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:2388317/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:248063/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:2518535/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:1506587/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:1505852/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:3352402/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:2082845/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:1501455/1‑69 (MQ=255) ggAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGc > 1:3193797/1‑69 (MQ=255) tGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATc < 1:2704712/69‑1 (MQ=255) tGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATc < 1:2528625/69‑1 (MQ=255) gCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCa > 1:1087728/1‑69 (MQ=255) cagcagCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCAcc > 1:444281/1‑67 (MQ=255) cagcagCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCAcc > 1:2377501/1‑67 (MQ=255) cagcagCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCAcc > 1:3243825/1‑67 (MQ=255) cagcagCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCAcc > 1:2253347/1‑67 (MQ=255) | TCCATCAGGCGTTTGTAACCCACCATGCCAATGGCAAAAGTGATATTGGAGAGTTGCACCAGCAGCAAGCCAGTCCAGAAATGGTCGGTGACCTGATCATAGCGAATAATCCCGGCACCAATCACC > minE/2387050‑2387175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |