| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,387,276 | G→A | 100% | L88L (CTG→TTG) | yigM ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,387,276 | 0 | G | A | 78.5% | 99.7 / 20.2 | 74 | L88L (CTG→TTG) | yigM | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (1/15); new base A (0/58); total (1/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACATCACGCCAAGCTGC > minE/2387243‑2387340 | tGATGTAGAGCGGCGTCAGCACGGTGAATAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:687213/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:1497744/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:2127697/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:2567623/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:160849/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:2816346/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:3304881/69‑1 (MQ=255)tGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGa < 1:1254154/69‑1 (MQ=255)ggATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTAAGGTAGAGATAAGCGCGGa < 1:3023697/68‑1 (MQ=255) gATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGaa < 1:144482/69‑1 (MQ=255) gATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGaa < 1:1455363/69‑1 (MQ=255) gATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGaa < 1:3372046/69‑1 (MQ=255) aTGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGaa < 1:777646/68‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:3285856/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:3199601/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:586046/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2497249/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2580579/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2593105/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:3318731/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2931961/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:3296891/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2946334/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2040935/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:1475026/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:1439067/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:907562/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:1285806/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:186312/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:1919035/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:1129986/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:107136/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:855906/69‑1 (MQ=255) tAGAGCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAAACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:2960582/69‑1 (MQ=255) agagCGGCGTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:703765/68‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2334987/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2966398/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1484913/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2934917/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1538051/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2913255/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1409859/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1970006/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2728310/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1587795/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:749984/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:3139214/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2265737/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:2420483/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:3149332/69‑1 (MQ=255) ggcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:1929907/69‑1 (MQ=255) gcgTCAGTACGGTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCata < 1:296868/68‑1 (MQ=255) tCAGCACGGTGCACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATAc > 1:1774981/1‑68 (MQ=255) cAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACAt < 1:2118656/69‑1 (MQ=255) aGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACAt < 1:2574987/68‑1 (MQ=255) ggTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTc < 1:697565/52‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:3421309/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:49005/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:856805/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:639143/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:682923/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:1090845/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:3028833/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:1540809/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:1660569/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:1971881/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2143158/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2228483/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2333842/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2438387/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2891555/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2917987/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:2989260/68‑1 (MQ=255) gTGAACAGCAACAGTTCGGAGACCGTCAGGTAGAGATAGGCGCGGAAACTCAGCATATACATCACAcc < 1:3093617/68‑1 (MQ=255) cagcagcagCTCGGAAACCGTCAGGTAGAGATAAg > 1:2337741/1‑35 (MQ=255) cagcagcagCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAg > 1:2282041/1‑48 (MQ=255) cagcagcagCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAg > 1:2272342/1‑48 (MQ=255) cagcagcagCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAg > 1:3356442/1‑48 (MQ=255) cagcagcagCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAg > 1:870048/1‑48 (MQ=255) agcagcagcTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACATCACGCCAAGCTGc < 1:2380414/69‑1 (MQ=255) agcagcagcTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACATCACGCCAAGCTGc < 1:922285/69‑1 (MQ=255) | TGATGTAGAGCGGCGTCAGCACGGTGAACAGCAGCAGCTCGGAAACCGTCAGGTAGAGATAAGCGCGGAAACTCAGCATATACATCACGCCAAGCTGC > minE/2387243‑2387340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |