Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,388,775 G→A 50.0% R223R (CGC→CGT)  pldB ← lysophospholipase L(2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,388,7750GA50.0% ‑3.6 / 95.0 102R223R (CGC→CGT) pldBlysophospholipase L(2)
Reads supporting (aligned to +/- strand):  ref base G (18/33);  new base A (26/25);  total (44/58)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 102 total aligned reads displayed.

TCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCAA  >  minE/2388716‑2388834
                                                           |                                                           
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCGGCATAGAAACGTAAGTTa                                                    >  1:1453460/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGtt                                                     >  1:1227604/1‑68 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1857279/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1929849/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2016520/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2199964/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1680355/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2227628/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2363098/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2607235/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1617425/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1490363/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:2824342/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:3143982/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:3300923/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1302603/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:3247375/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:964401/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1289032/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:444066/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1228273/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:878665/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:448312/1‑69 (MQ=255)
tCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAACGTAAGTTa                                                    >  1:1209492/1‑69 (MQ=255)
      aCCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              <  1:3199286/69‑1 (MQ=255)
      aCCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              <  1:1996100/69‑1 (MQ=255)
      aCCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              <  1:769101/69‑1 (MQ=255)
                   tCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTctgc                                 <  1:2198454/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:2561394/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:670886/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:2454877/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:250176/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:2680950/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:343490/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:3045338/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:1638653/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:1529543/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:1280223/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 <  1:1381894/69‑1 (MQ=255)
                                   aaTCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTATCTGCTGTGGGTCAGTACGtt                 <  1:1400240/69‑1 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:364290/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:2597148/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:66927/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:651173/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:1216412/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:3215820/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:876260/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:2004924/1‑67 (MQ=255)
                                     tCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGtt                 >  1:1948854/1‑67 (MQ=255)
                                       gTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              >  1:3144715/1‑36 (MQ=255)
                                       gTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              >  1:2422527/1‑36 (MQ=255)
                                       gTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              >  1:1468625/1‑36 (MQ=255)
                                       gTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGa                                              >  1:397989/1‑36 (MQ=255)
                                         tgtgTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1082353/65‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCTATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:30853/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2632578/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1840119/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1305476/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:957524/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1374442/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1719451/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1174565/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2346396/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1669810/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:3107378/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:3086530/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1659023/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:3048295/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2487026/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1641882/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1190045/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:1056113/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2793863/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:278750/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:690943/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2594836/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAg            <  1:2596291/68‑1 (MQ=255)
                                         tGGGTCATCAGCATAGAAACGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTCATAg            <  1:2818656/68‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:50294/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:836597/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:673871/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:902075/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:3405483/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:322976/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:3080959/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:1461332/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:960553/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:2918314/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:278236/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:2717182/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:1673525/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:1712146/69‑1 (MQ=255)
                                          gggTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCa          <  1:1081840/69‑1 (MQ=255)
                                                 caGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGc    <  1:190130/68‑1 (MQ=255)
                                                 caGCATAGAAGCGTAAGTTACGTCGATATCGCTGTATGCTGTGGGTCAGTACGTTGATAGCAAACGGc    <  1:1289186/68‑1 (MQ=255)
                                                  aGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCtgtg                             >  1:1710722/1‑42 (MQ=255)
                                                  aGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCaa  >  1:3060305/1‑69 (MQ=255)
                                                  aGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCaa  >  1:3270706/1‑69 (MQ=255)
                                                  aGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCaa  >  1:2989232/1‑69 (MQ=255)
                                                  aGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCaa  >  1:185336/1‑69 (MQ=255)
                                                           |                                                           
TCGCGTACCCAATGGTAGGTCGGCCCACCGACGCGAATCGTTGGGTCATCAGCATAGAAGCGTAAGTTACGTCGATATCGCTGTCTGCTGTGGGTCAGTACGTTGATAGCAAACGGCAA  >  minE/2388716‑2388834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑