| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,390,896 | A→C | 41.1% | intergenic (‑40/+24) | rhtC ← / ← recQ | threonine efflux system/ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,390,896 | 0 | A | C | 41.1% | 28.1 / 91.0 | 90 | intergenic (‑40/+24) | rhtC/recQ | threonine efflux system/ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (35/18); new base C (19/18); total (54/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATGCGCACGAATCAGCGC > minE/2390849‑2390961 | aTCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATgctgct < 1:2732886/69‑1 (MQ=255)aTCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATgctgct < 1:3324295/69‑1 (MQ=255)aTCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATgctgct < 1:618180/69‑1 (MQ=255)aTCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATgctgct < 1:2904625/69‑1 (MQ=255) tCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGctgctg > 1:484476/1‑69 (MQ=255) tCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGctgctg > 1:361256/1‑69 (MQ=255) tCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGctgctg > 1:1243244/1‑69 (MQ=255) acatTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActc < 1:77795/69‑1 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACTGAGTCTTCATACTGGCACtt < 1:1409689/48‑1 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACtt < 1:652293/48‑1 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACtt < 1:132438/48‑1 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACtt < 1:2851151/48‑1 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1951914/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1926335/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1861147/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1827340/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:3351205/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:170873/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:2465081/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1048406/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:1407206/1‑69 (MQ=255) catTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActct > 1:859364/1‑69 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1511002/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1124138/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1149018/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1511580/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:233302/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1524841/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:2676411/61‑1 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2768579/1‑69 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2839371/1‑69 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:1538002/1‑69 (MQ=255) tttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:422132/1‑69 (MQ=255) ttGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGac < 1:1856912/60‑1 (MQ=255) aCTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTActc < 1:85265/62‑1 (MQ=255) tCGCGGGGGAAACGTTTACTGAGTCTTCATCCTGGCACttttt < 1:1497198/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:999840/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:308804/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:530360/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:3255711/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:3375435/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:3425846/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:49143/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:375274/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:1178287/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:1874672/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:2161465/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:2312201/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:1121636/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:2334265/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:2367108/43‑1 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2590349/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2488469/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2848778/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2393053/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:2946862/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:1955334/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:3098376/1‑63 (MQ=255) tCGCGGGGGAAACGTTTACGGAGTCTTCATCCTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:1025290/1‑63 (MQ=255) cgcgGGGGAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:977008/42‑1 (MQ=255) ggggAAACGTTTACGGAGTCTTCATCCTGGCACttttt < 1:2986153/38‑1 (MQ=255) aaaCGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGact > 1:1331710/1‑47 (MQ=255) aaaCGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAAc > 1:2441281/1‑69 (MQ=255) aaaCGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAAc > 1:1544351/1‑69 (MQ=255) aaaCGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAAc > 1:170863/1‑69 (MQ=255) aaaCGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAAc > 1:3019763/1‑69 (MQ=255) tttACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcg > 1:2432355/1‑69 (MQ=255) tttACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcg > 1:1662139/1‑69 (MQ=255) tttACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcg > 1:943628/1‑69 (MQ=255) tttACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcg > 1:1530573/1‑69 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATgctgct > 1:976311/1‑36 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTc > 1:1790314/1‑47 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:2116677/1‑68 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:2704554/1‑68 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:1100287/1‑68 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:579437/1‑68 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:3024404/1‑68 (MQ=255) tACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATgcgc > 1:1805251/1‑68 (MQ=255) gTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATGCGCACGAATc > 1:1652462/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:608335/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:1852169/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:667049/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:2734851/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:834595/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:872772/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcgc > 1:1776226/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcg > 1:2216928/1‑68 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACATGCGCACGAATCAgcg > 1:1354551/1‑68 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATCGACAAGCGCACGAATCAgcgc > 1:1054163/1‑69 (MQ=255) cATCCTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCGTCACCATAGACATGCGCACGAATCAgcgc > 1:857258/1‑69 (MQ=255) | ATCAACATACATTTGACTCGCGGGGGAAACGTTTACGGAGTCTTCATACTGGCACTTTTTTATGCTGCTGACTACTCTTCGTCATCGCCATCAACATGCGCACGAATCAGCGC > minE/2390849‑2390961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |