| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,392,399 | A→G | 42.3% | R117R (CGT→CGC) | recQ ← | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,392,399 | 0 | A | G | 42.3% | 11.7 / 37.1 | 52 | R117R (CGT→CGC) | recQ | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base A (7/23); new base G (13/9); total (20/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01 | |||||||||||
GAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAG > minE/2392356‑2392462 | gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:3230051/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1922180/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1530825/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:429842/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1267342/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:386287/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1165246/68‑1 (MQ=255)gAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1072307/68‑1 (MQ=255) aaaGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1161409/67‑1 (MQ=255) gTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:3134855/69‑1 (MQ=255) gTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:2125211/69‑1 (MQ=255) gTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:829647/69‑1 (MQ=255) gTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:582400/69‑1 (MQ=255) gTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:1834088/69‑1 (MQ=255) aTCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:2966266/66‑1 (MQ=255) gCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCTGTGCGGCAGCCTGTCATCACTTCa < 1:2667319/68‑1 (MQ=255) gCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCa < 1:1374844/68‑1 (MQ=255) aTCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGt < 1:1898191/69‑1 (MQ=255) gTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCt < 1:1521219/47‑1 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGtt > 1:103139/1‑60 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTcgc > 1:1839030/1‑69 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTcg > 1:1438693/1‑68 (MQ=255) ggCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGtcat < 1:3183976/45‑1 (MQ=255) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:625862/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:1709328/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:850148/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2216265/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2297142/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:533546/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2469293/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:125194/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:1051625/44‑1 (MQ=37) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:3291360/53‑1 (MQ=255) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:1027922/53‑1 (MQ=255) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:3150245/53‑1 (MQ=255) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:932623/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:3272475/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:915762/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:446186/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2834064/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2637894/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2300373/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2073011/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1773536/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1663919/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:166365/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1549638/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1199863/1‑43 (MQ=37) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGa > 1:2872380/1‑66 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:3048752/1‑67 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:3283738/1‑67 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:2780692/1‑67 (MQ=255) | GAAAGTTATCCAGCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAG > minE/2392356‑2392462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |