| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,392,429 | C→T | 38.5% | V107V (GTG→GTA) | recQ ← | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,392,429 | 0 | C | T | 38.5% | 29.3 / 34.6 | 57 | V107V (GTG→GTA) | recQ | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base C (24/11); new base T (13/9); total (37/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAG > minE/2392368‑2392497 | gCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCTGTGCGGCAGCCTGTCATCACTTCa < 1:2667319/68‑1 (MQ=255)gCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCa < 1:1374844/68‑1 (MQ=255) aTCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGt < 1:1898191/69‑1 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGtt > 1:103139/1‑60 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTcgc > 1:1839030/1‑69 (MQ=255) ccGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTcg > 1:1438693/1‑68 (MQ=255) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2297142/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2216265/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:2469293/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:1709328/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:533546/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:625862/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:850148/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:125194/44‑1 (MQ=37) taAAGCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTc < 1:1051625/44‑1 (MQ=37) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:3291360/53‑1 (MQ=255) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:3150245/53‑1 (MQ=255) taAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTg < 1:1027922/53‑1 (MQ=255) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:446186/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:3272475/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2834064/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:915762/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2637894/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2300373/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:932623/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:2073011/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1199863/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1549638/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:166365/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1663919/1‑43 (MQ=37) gCAGGCGGATTTGCCCGGTGCGGCAGCCTGTCATTACTTCAAgc > 1:1773536/1‑43 (MQ=37) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGa > 1:2872380/1‑66 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:2780692/1‑67 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:3283738/1‑67 (MQ=255) cAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAg > 1:3048752/1‑67 (MQ=255) ggTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgccgc < 1:842343/69‑1 (MQ=255) ggTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgccgc < 1:3436380/69‑1 (MQ=255) ggTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgccgc < 1:3416459/69‑1 (MQ=255) ggTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgccgc < 1:2874389/69‑1 (MQ=255) ggTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgccgc < 1:243827/69‑1 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCa > 1:1552375/1‑68 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:2613751/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:2269798/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:345405/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:2382050/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:2773619/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:2026672/1‑69 (MQ=255) gCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCAc > 1:321252/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCAcgccgcc > 1:2850610/1‑55 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:1186155/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:1693645/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:3301116/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:2802980/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:2513814/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:2346753/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:1533905/1‑69 (MQ=255) tGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGc > 1:1504806/1‑69 (MQ=255) caCTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCa > 1:346887/1‑68 (MQ=255) caCTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAg > 1:2000842/1‑69 (MQ=255) caCTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAg > 1:1421468/1‑69 (MQ=255) caCTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAg > 1:350562/1‑69 (MQ=255) caCTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAg > 1:3178517/1‑69 (MQ=255) | GCATCAGGCGTTCCGGGGCGATATAAAGCAGACGAATTTGCCCGGTGCGGCAGCCTGTCATCACTTCAAGTTGCTGTTCGCGGGTTTGCGTCGAGTTAAGGCACGCCGCCGCCACGCCGTTGGCTTGCAG > minE/2392368‑2392497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |