| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,393,275 | C→G | 100% | G159G (GGG→GGC) | pldA ← | outer membrane phospholipase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,393,275 | 0 | C | G | 83.4% | 58.4 / 5.5 | 30 | G159G (GGG→GGC) | pldA | outer membrane phospholipase A |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base G (8/17); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGGCGGTTCCAGCTGCGGGAGGTCGGGTCGGAACGCCCGTTAGAGTCGTGGTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGA > minE/2393218‑2393336 | aaGGCGGTTCCAGCTGCGGGAGGTCGGGTCGGAACGCCCGTTAGAGTCGTGGTTATACCCCATCTCca < 1:1640940/68‑1 (MQ=255)aaGGCGGTTCCAGCTGCGGGAGGTCGGGTCGGAACGCCCGTTAGAGTCGTGGGTATAaccc > 1:910913/1‑61 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTcc > 1:686892/1‑56 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCgcgc > 1:564188/1‑65 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:2607814/1‑69 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:975535/1‑69 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:954261/1‑69 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:1042924/1‑69 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:1933345/1‑69 (MQ=255) aGCTGCGGGAAGTCGGGTCTGAACGCCCGTTTGAGTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCg > 1:1733921/1‑69 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:1989989/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:1237532/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:1457671/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:709688/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:693219/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:3345300/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:2865636/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:2603235/68‑1 (MQ=255) tgagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:2521861/68‑1 (MQ=255) gagTCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCaaa < 1:859031/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:2627825/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:3096281/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:3100300/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:1732805/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:1714971/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:2598970/68‑1 (MQ=255) tCGTGGTTGTAGCCCATTTCCACATCGCGCAGCGTCCAGCCAGCAAAGTTGTAATCCGTGGCAAAAcc < 1:1628669/68‑1 (MQ=255) gTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGa > 1:3276127/1‑68 (MQ=255) gTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGa > 1:3387111/1‑68 (MQ=255) gTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGa > 1:2254978/1‑68 (MQ=255) gTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGa > 1:1993437/1‑68 (MQ=255) | AAGGCGGTTCCAGCTGCGGGAGGTCGGGTCGGAACGCCCGTTAGAGTCGTGGTTATACCCCATCTCCACATCGCGCAGCGTCCAACCTGCAAAACGGTAATCGGTGGCAAAACCGAGGA > minE/2393218‑2393336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |