| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,394,275 | T→C | 100% | T120T (ACT→ACC) | yigI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,394,275 | 0 | T | C | 76.6% | 17.3 / 0.8 | 17 | T120T (ACT→ACC) | yigI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base C (13/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGGACCATTGATCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAG > minE/2394212‑2394341 | ggggACCATTGATCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACtagtag > 1:839114/1‑69 (MQ=255)ggggACCATTGATCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACtagtag > 1:2596000/1‑69 (MQ=255) gATCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCg > 1:3060145/1‑68 (MQ=255) tCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCGTg < 1:566576/68‑1 (MQ=255) tCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCGTg < 1:2005835/68‑1 (MQ=255) ggggCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTg < 1:997114/69‑1 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:2743944/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:852917/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:634851/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:32581/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:312267/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:2972406/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:2483347/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:1229607/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:1233507/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:2159238/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:138585/1‑66 (MQ=255) tACTGCTACCAGTAGCCTGTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:1518260/1‑66 (MQ=255) tACTGCTACCAGTAGCCTCTTACGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACaa > 1:545158/1‑66 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCGGTCGCCCGCGTTGAATTACACAATGAAGAACa < 1:2308597/68‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAagag < 1:989419/69‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACa < 1:118217/68‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACa < 1:3153127/68‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACa < 1:1059557/68‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:1700482/69‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:2009711/69‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:2769746/69‑1 (MQ=255) actaGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:2743705/69‑1 (MQ=255) actaGTAGCCTGTTGCGTACAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:1590863/69‑1 (MQ=255) actaGTAGCCTGTTGCGTACAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAg < 1:2849396/69‑1 (MQ=255) | GGGGACCATTGATCTTCGCGTTGATTATCTGCGCCCAGGCAGGGGCGAGCGTTTTACTGCTACTAGTAGCCTGTTGCGTGCAGGCAATAAAGTCGCCGTCGCCCGCGTTGAATTACACAATGAAGAACAG > minE/2394212‑2394341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |