| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 197,781 | T→C | 31.4% | L213L (CTA→CTG) | yaeB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 197,781 | 0 | T | C | 31.4% | 96.7 / 56.2 | 111 | L213L (CTA→CTG) | yaeB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/60); new base C (26/9); total (42/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTG > minE/197713‑197843 | tAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCTAACGTTAAAATCATGt < 1:2525548/69‑1 (MQ=255)tAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGt < 1:1987976/69‑1 (MQ=255)tAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGt < 1:498527/69‑1 (MQ=255) aCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa < 1:3192485/68‑1 (MQ=255) aCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa < 1:802249/68‑1 (MQ=255) aCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTa < 1:1292755/68‑1 (MQ=255) gcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc < 1:3120160/68‑1 (MQ=255) gcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc < 1:2028597/68‑1 (MQ=255) gcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc < 1:1160068/68‑1 (MQ=255) gcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc < 1:1178177/68‑1 (MQ=255) gcgGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGc < 1:1287406/68‑1 (MQ=255) gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGa > 1:3084774/1‑69 (MQ=255) ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa > 1:51386/1‑64 (MQ=255) ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACgg > 1:2335505/1‑69 (MQ=255) ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACgg > 1:526262/1‑69 (MQ=255) ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACgg > 1:1101660/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:38741/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:2143731/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:3216185/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:530204/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:2025784/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:1063684/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:537904/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:607216/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:1846094/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:1290502/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:9362/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:1135056/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGACAGACGGCATAGGttt > 1:2206837/1‑69 (MQ=255) aGACTTCAAAACCTGCGTCGGTGACGCGCCAGAGAACGTTAAAATCATGCAGCCAGACGGCATAGGttt > 1:1458729/1‑69 (MQ=255) aGACTT‑AAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGtttt > 1:2275266/1‑69 (MQ=255) ttCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:100808/69‑1 (MQ=255) ttCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:409108/69‑1 (MQ=255) ttCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:10689/69‑1 (MQ=255) ttCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:552313/69‑1 (MQ=255) ttCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:568055/69‑1 (MQ=255) tCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:2836772/68‑1 (MQ=255) tCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:1256891/68‑1 (MQ=255) cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa > 1:2667504/1‑49 (MQ=255) cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa > 1:2212887/1‑49 (MQ=255) cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa > 1:2663428/1‑49 (MQ=255) cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCa > 1:2499487/1‑49 (MQ=255) aaCCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGtt < 1:1663552/68‑1 (MQ=255) aaCCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGtt < 1:3212755/68‑1 (MQ=255) aaCCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGtt < 1:1132623/68‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:2632406/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:256240/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:3075612/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:2239556/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:630124/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:614788/69‑1 (MQ=255) cTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:1844073/69‑1 (MQ=255) tGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc < 1:2428841/69‑1 (MQ=255) gCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc < 1:2513220/68‑1 (MQ=255) gCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc < 1:2464559/68‑1 (MQ=255) gCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTc < 1:2059048/68‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGGCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:607588/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTTCCCGTTTCCTCACCTTTAc < 1:2912851/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:867869/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:3303016/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:774595/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:951402/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:556109/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:3032893/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:1819877/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:2253271/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:2375047/69‑1 (MQ=255) tGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:2591097/69‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:2810107/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:2814051/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:1934767/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:3192275/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:2903600/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:2136920/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:806332/51‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:909396/68‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAc < 1:1942780/68‑1 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTAACCTTTAc > 1:1746541/1‑68 (MQ=255) gACGCGCCAGCGAACGTTAAAATCATGTAACCAGACGGCATAGGTTTTGcc < 1:2653384/51‑1 (MQ=255) gcgcCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGcc < 1:731675/48‑1 (MQ=255) gcgcCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa > 1:918881/1‑69 (MQ=255) gcgcCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa > 1:1392475/1‑69 (MQ=255) gcgcCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATa > 1:2482588/1‑69 (MQ=255) gCGAACGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCt < 1:460744/50‑1 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTAGGATAGGCCGGGcgcg > 1:350308/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:1568453/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:1689340/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:1276323/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:1882343/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:1910092/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:2086863/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:512903/1‑69 (MQ=255) cGTTAAAATCATGCAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGcgcg > 1:2707021/1‑69 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:689142/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:556258/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:1681537/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:1936996/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:3266615/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:1306292/69‑1 (MQ=255) aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTg < 1:291260/69‑1 (MQ=255) | TAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTG > minE/197713‑197843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |