| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,399,893 | G→A | 48.2% | V246V (GTC→GTT) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,399,893 | 0 | G | A | 48.2% | 0.9 / 46.7 | 56 | V246V (GTC→GTT) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base G (18/11); new base A (18/9); total (36/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCG > minE/2399830‑2399941 | gAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACcgacga > 1:2585958/1‑68 (MQ=255)gAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGat > 1:1031458/1‑69 (MQ=255)gAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGat > 1:2673898/1‑69 (MQ=255)gAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGat > 1:3237309/1‑69 (MQ=255) aaaCGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGatc > 1:1318700/1‑69 (MQ=255) aaaCGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGat > 1:523266/1‑68 (MQ=255) cTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCAc < 1:2238317/69‑1 (MQ=255) cTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCAc < 1:1500292/69‑1 (MQ=255) cTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCAc < 1:1603919/69‑1 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:2535383/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:3047816/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:385326/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:55108/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:736563/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:1037452/1‑69 (MQ=255) tatTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGc > 1:908462/1‑69 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc < 1:1637879/68‑1 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc < 1:34626/68‑1 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc < 1:519158/68‑1 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc < 1:3169812/68‑1 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc < 1:2674067/68‑1 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCg > 1:2831923/1‑69 (MQ=255) ttCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCg > 1:1019677/1‑69 (MQ=255) gcgcCATCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2225/1‑57 (MQ=37) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAgtat > 1:9093/1‑54 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAgtat > 1:3131803/1‑54 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1397561/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:352519/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1795127/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3360753/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2558791/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1520159/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3136046/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:213105/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3004999/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:137875/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2850536/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1312417/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2413018/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:246978/1‑57 (MQ=38) gcgcCAGCCGTAGATTGACTGGTCGTCATAGCCAACGATCATCACTTTGCCAGTATc > 1:2603160/1‑57 (MQ=255) ccGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATc < 1:2337523/69‑1 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGcc > 1:1367643/1‑42 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTcgcc > 1:1862475/1‑51 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:3225176/1‑69 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:2613707/1‑69 (MQ=255) ttGACTGGTCGTCATCGGCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2000434/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:410985/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2989401/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:521089/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2985619/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2785107/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2690590/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:92951/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:944340/68‑1 (MQ=255) ttGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:1019526/68‑1 (MQ=255) tGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:613475/67‑1 (MQ=255) | GAAACGCTGAATATTCTCCACCTGCGCCCCGCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCG > minE/2399830‑2399941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |