| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,399,914 | G→A | 52.1% | D239D (GAC→GAT) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,399,914 | 0 | G | A | 52.1% | ‑3.0 / 40.8 | 48 | D239D (GAC→GAT) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base G (11/12); new base A (16/9); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGAT > minE/2399860‑2399982 | gcgcCATCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2225/1‑57 (MQ=37)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1795127/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2558791/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:246978/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2413018/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:213105/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:2850536/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3004999/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1520159/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3136046/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1397561/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:137875/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:3360753/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:1312417/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATc > 1:352519/1‑57 (MQ=38)gcgcCAGCCGTAGATTGACTGGTCGTCATAGCCAACGATCATCACTTTGCCAGTATc > 1:2603160/1‑57 (MQ=255) ccGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATc < 1:2337523/69‑1 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTcgcc > 1:1862475/1‑51 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:3225176/1‑69 (MQ=255) gTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCa > 1:2613707/1‑69 (MQ=255) ttGACTGGTCGTCATCGGCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2000434/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:92951/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:521089/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:410985/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2690590/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2785107/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2985619/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:944340/68‑1 (MQ=255) ttGACTGGTCGTCATCGCCAACGATCATCACTTTGCCAGTATCGCCTGCCAGCAGTCGGATCCAcgcg < 1:2989401/68‑1 (MQ=255) ttGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:1019526/68‑1 (MQ=255) tGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCAcgcg < 1:613475/67‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTTTCCTgg < 1:434955/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:2312378/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:76924/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:3187805/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1585506/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1986383/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTgg < 1:1623109/68‑1 (MQ=255) tcatcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTCTTGGTATCCTgg < 1:3304181/68‑1 (MQ=255) catcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGaa > 1:1106116/1‑69 (MQ=255) catcaCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGaa > 1:1199722/1‑69 (MQ=255) aCTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCg > 1:407780/1‑69 (MQ=255) ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:86416/1‑69 (MQ=255) ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:882514/1‑69 (MQ=255) ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:94018/1‑69 (MQ=255) ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:1317840/1‑69 (MQ=255) ttGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTcc > 1:2947996/1‑69 (MQ=255) ggTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAg < 1:3009008/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:3380091/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:3278725/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:1445531/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:1111435/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:1032954/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:1466941/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:2081380/69‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGa < 1:2081626/69‑1 (MQ=255) gtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGat > 1:2437741/1‑69 (MQ=255) gtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGat > 1:718807/1‑69 (MQ=255) gtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGat < 1:1994070/69‑1 (MQ=255) gtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGat < 1:321087/69‑1 (MQ=255) gtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTAGTCCACCAGGat > 1:371367/1‑69 (MQ=255) | GCGCCAGCCGTAGATTGACTGGTCGTCATCACCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGGTATCCTGGAATTCGTCCACCAGGAT > minE/2399860‑2399982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |