| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,401,943 | A→G | 31.2% | N128N (AAT→AAC) | xerC ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,401,943 | 0 | A | G | 31.2% | 68.2 / 39.5 | 80 | N128N (AAT→AAC) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base A (7/48); new base G (2/23); total (9/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
AAAGACGCAGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCA > minE/2401879‑2401998 | aaaGACGCAGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGa < 1:2727970/69‑1 (MQ=255)aaaGACGCAGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGa < 1:2860462/69‑1 (MQ=255) aaGACGCAGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGa < 1:2899252/68‑1 (MQ=255) aGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAata < 1:2066586/69‑1 (MQ=255) aGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAata < 1:575009/69‑1 (MQ=255) aGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAata < 1:716583/69‑1 (MQ=255) gACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGa < 1:264465/60‑1 (MQ=255) cccGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATcc < 1:873463/69‑1 (MQ=255) cccGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATcc < 1:3397887/69‑1 (MQ=255) cccGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAAGGGATCATTGATATCAATATcc < 1:2704612/69‑1 (MQ=255) tACATCACTTCCAGCATTGCACGGTCGCGTAGAGCGAGGGGATCATTGATATc > 1:735111/1‑53 (MQ=255) tACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATc > 1:1389035/1‑53 (MQ=255) aCATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCa > 1:860320/1‑44 (MQ=255) cACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:558451/69‑1 (MQ=255) cACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:3319117/69‑1 (MQ=255) cACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:3330411/69‑1 (MQ=255) cACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:117692/69‑1 (MQ=255) cACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:1196829/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCATCAGCCGATTCata < 1:3113142/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1171565/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:255689/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:263712/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1031034/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1132720/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1141571/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1161094/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1986483/69‑1 (MQ=255) aCTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1430639/69‑1 (MQ=255) aCTTCCAGCAATGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1335783/69‑1 (MQ=255) cTTCCAGCATTGCACGGTCGCGTACAGCGAGGTGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:116398/67‑1 (MQ=255) cTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTCatat > 1:2045813/1‑69 (MQ=255) cTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTCatat > 1:2587915/1‑69 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:3080564/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:421105/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:3162830/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:2676499/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:233790/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:519839/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:3435139/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:2400859/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:2363378/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:16206/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:2232678/68‑1 (MQ=255) aGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATAtcgtc < 1:164955/68‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2041331/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:1064346/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:524361/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:1464444/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:517273/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:1471010/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:1575708/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:3283104/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:3239048/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:3238917/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:1739326/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:669855/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:625571/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:3016737/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2976418/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2970596/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2082523/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2846833/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2258056/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2292233/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:58655/54‑1 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCATTGATATc > 1:22897/1‑35 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGAtt > 1:1692787/1‑53 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGAtt > 1:166192/1‑53 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGAtt > 1:2589829/1‑53 (MQ=255) gCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATcgtcgacgtc > 1:718295/1‑68 (MQ=255) gCACGGTCGCGCACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:2435765/54‑1 (MQ=39) cACGGTCGCGTACAGCGAGGGGATCGTTGATATCAATATCCAGCAGCCGATTc < 1:3037960/53‑1 (MQ=255) tCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:1425852/50‑1 (MQ=255) gTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCat < 1:201315/46‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:721251/69‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:90262/69‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:2570851/69‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:2045645/69‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:2033420/69‑1 (MQ=255) cAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCa < 1:1435830/69‑1 (MQ=255) gATCATTGATATCAATATCCAGCAGCCGATTCata < 1:1104525/35‑1 (MQ=255) | AAAGACGCAGACCCGCGCCGTACATCACTTCCAGCATTGCACGGTCGCGTACAGCGAGGGGATCATTGATATCAATATCCAGCAGCCGATTCATATCGTCGACGTCGATGTTTTTCGGCA > minE/2401879‑2401998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |