| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,402,395 | T→C | 40.8% | Q212Q (CAA→CAG) | yigA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,402,395 | 0 | T | C | 40.8% | 34.3 / 67.7 | 93 | Q212Q (CAA→CAG) | yigA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (29/26); new base C (15/23); total (44/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
CGCGTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCATCGCTT > minE/2402328‑2402461 | cgcgTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTAATGAAGTAACTGCGTTCCtt > 1:1344312/1‑69 (MQ=255) cgTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGc > 1:1139668/1‑69 (MQ=255) cgTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGc > 1:388979/1‑69 (MQ=255) cgTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGc > 1:1558144/1‑69 (MQ=255) cAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCtt > 1:2231019/1‑69 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1490004/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2371280/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1990617/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:250249/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2661029/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2784217/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2799197/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1804009/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:3000112/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1509107/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:3214307/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1063523/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:142211/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:930876/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:3425948/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1109014/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:1277398/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:3303437/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:656702/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:676312/63‑1 (MQ=255) ccAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:756500/63‑1 (MQ=255) cAACGCGCCAGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2256177/62‑1 (MQ=255) cTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:534779/69‑1 (MQ=255) cTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:2348189/69‑1 (MQ=255) cTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:2949501/69‑1 (MQ=255) cTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:154638/69‑1 (MQ=255) cTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:1471516/69‑1 (MQ=255) cAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTg < 1:3120304/66‑1 (MQ=255) aGAAGCTCCGGCAACATCAGTGCAATTTCATGAAGTAACTGCGTTCCCTGccc < 1:2521264/53‑1 (MQ=255) tCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGGCTGGTa > 1:996053/1‑68 (MQ=255) aGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAAc > 1:2901077/1‑69 (MQ=255) aGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAAc > 1:1645323/1‑69 (MQ=255) cgcAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:795040/1‑69 (MQ=255) cgcAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:314355/1‑69 (MQ=255) cgcAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:445965/1‑69 (MQ=255) cgcAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:450071/1‑69 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCGTCGCGACTGGTAAACAg > 1:2251982/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:540843/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:1223965/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:686334/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:254281/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:2611121/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:40606/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:1472575/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:330088/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:2709333/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:2759328/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:2149421/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:1590231/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:1059849/1‑68 (MQ=255) gcAATTTCATGAAGTAACTGCGTTCCCTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAg > 1:3000111/1‑68 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGTTAAACAGCACGa < 1:2166656/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:3085014/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:1974331/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:311932/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:401976/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:3154642/69‑1 (MQ=255) tttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:3196935/69‑1 (MQ=255) ttCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGa < 1:2694219/68‑1 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:1661600/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:2275202/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:994550/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:2301687/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:2119330/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:2649587/1‑69 (MQ=255) tCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGaca > 1:3256666/1‑69 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTTGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:1970506/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTa > 1:1485201/1‑47 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:496715/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:293244/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:296444/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:73192/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:1161321/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:2398886/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:2458002/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:2796373/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAg < 1:1389181/69‑1 (MQ=255) aCTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACCACACCCAAATCAg < 1:3118693/69‑1 (MQ=255) aCTGCGTTCCTTGCCATTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCAAAATCAg < 1:1930220/69‑1 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGc > 1:2799934/1‑68 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:1939164/1‑69 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:1273166/1‑69 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:114406/1‑69 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:1086048/1‑69 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:1965809/1‑69 (MQ=255) tGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCa > 1:314754/1‑69 (MQ=255) cGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCATc > 1:1338002/1‑69 (MQ=255) cTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCATCGCtt > 1:3376611/1‑68 (MQ=255) cTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCATCGCtt > 1:3372514/1‑68 (MQ=255) | CGCGTTCAATCCAACGCTCCAGAAGCTCCGGCAACATCAGCGCAATTTCATGAAGTAACTGCGTTCCTTGCCCTTGTTGATAGTGACTGGCATCGCGACTGGTAAACAGCACGACACCCAAATCAGCATCGCTT > minE/2402328‑2402461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |