| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 198,312 | C→T | 100% | L36L (CTG→CTA) | yaeB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 198,312 | 0 | C | T | 84.8% | 39.1 / 0.3 | 26 | L36L (CTG→CTA) | yaeB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (0/22); total (2/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGA > minE/198249‑198380 | tAAATGGCTGAATGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:2523098/69‑1 (MQ=255)tAAATGGCTGAATGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:882485/69‑1 (MQ=255)tAAATGGCTGAATGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:646862/69‑1 (MQ=255)tAAATGGCTGAATGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:3057363/69‑1 (MQ=255)tAAATGGCTGAATGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:2413981/69‑1 (MQ=255)tAAATGGCTGAATGCTTCCAGGCCGCCAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTc < 1:3194108/69‑1 (MQ=255) cTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCtt > 1:1537832/1‑68 (MQ=255) cTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCtt > 1:864813/1‑68 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:104876/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:87788/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:779241/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:647620/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:3305536/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:3275480/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:2797207/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:1017390/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:1489015/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:2345151/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:2210141/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:2124744/69‑1 (MQ=255) tCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:1990123/69‑1 (MQ=255) tCCAGGCCGCGAAAGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:1955239/69‑1 (MQ=255) gCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAg < 1:2051064/69‑1 (MQ=255) gcgAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:1127510/61‑1 (MQ=255) tAGGGAGCAATGAGATGTAGTTCGCCGTTGGCGCttttt < 1:678444/39‑1 (MQ=255) cAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCtt < 1:3436491/69‑1 (MQ=255) cAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCgg > 1:3373515/1‑42 (MQ=255) cAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGgcga > 1:1273873/1‑69 (MQ=255) cAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGgcga > 1:1939496/1‑69 (MQ=255) | TAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGGCGCTTTTTACCAGACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGA > minE/198249‑198380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |